SN/T 4260-2015 English PDF (SN/T4260-2015)

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SN/T 4260-2015: Determination of crude polysaccharides in plant source foods for export. Phehol-sulfuric acid colorimetry
SN/T 4260-2015
(Export food of plant origin - Determination of polysaccharides in phenol - sulfuric acid method)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Export of foods of plant origin polysaccharide
Determination of phenol - sulfuric acid method
Issued on. 2015-05-26
2016-01-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Ningxia Exit Inspection and Quarantine.
The main drafters of this standard. Sun Min, Li Ping, Haojun Hu, Sun Pubing, Wynn Yan, Chen Lin, Yang Xuguang, Li Zhu Yan.
Export of foods of plant origin polysaccharide
Determination of phenol - sulfuric acid method
1 Scope
This standard specifies the export of plant-derived foods polysaccharides content colorimetric assay.
This standard applies to the determination of the content of edible fungus polysaccharides, Chinese wolfberry, grapes, jujube, fruit juice and other plant-derived foods.
This standard does not add starch, dextrin food components.
The detection limit of the standard method of 0.5mg/kg.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682-2008 Analysis
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Polysaccharide crudepolysaccharides
Β-D- glucan with α-D- glucan or other carbon sugar or a series of main chain polymer compound. Molecular formula (C6H12O6) n.
4 Method summary
Polysaccharides under the action of concentrated sulfuric acid hydrolysis to monosaccharides, uronic and rapid dehydration derivative, is reacted with a phenol to generate orange solution,
Characteristic absorption at 490nm, a quantitative comparison with the standard series.
5 Reagents and materials
Unless otherwise specified, use only analytical reagents, test water should be consistent with the three water GB/T 6682-2008 stipulated.
5.1 sulfate (H2SO4), ρ = 1.84g/mL.
5.2 absolute ethanol (C2H6O).
5.3 Phenol (C6H6O), redistilled.
5.4 80% ethanol solution.
5.5 glucose (C6H12O6), before use at 105 ℃ temperature drying to constant weight.
5.6 80% phenol solution. Weigh 80g of phenol (5.3) in the 100mL beaker, dissolved in water, go to the 100mL flask set brown
Yung, at 4 ℃ refrigerator stored.
5.7 5% phenol solution. lessons 5mL phenol solution (5.6), was dissolved in 75mL of water, mix, using now.
5.8 100mg/L glucose standard solution. Weigh 0.100g glucose (5.5) in the 100mL beaker, dissolved in water, dilute to