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QB/T 2881-2013 English PDF (QBT2881-2013)

QB/T 2881-2013 English PDF (QBT2881-2013)

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QB/T 2881-2013: Footwear and footwear components - Antimicrobial performance specifications

This standard specifies the terms and definitions, test environment, sampling, test strains, test method, requirements, determination, identification, and report of antibacterial performances of footwear and footwear components. This standard applies to footwear and footwear components with antibacterial performance.
QB/T 2881-2003
ICS 61.060
Y 28
File number. 43565-2013
Replacing QB/T 2881-2007
Footwear and footwear components -
Antibacterial performance specifications
Issued by. Ministry of Industry and Information Technology of the
People's Republic of China
Table of Contents
Foreword ... 3
1 Scope ... 5
2 Normative references ... 5
3 Terms and definitions ... 5
4 Test environment ... 6
5 Sampling ... 6
6 Test strains ... 6
7 Test methods ... 6
8 Requirements ... 7
9 Judgment ... 8
10 Identification ... 8
11 Report ... 8
Annex A ... 9
Annex B ... 12
Annex C ... 17
Annex D ... 20
Annex E ... 23
This standard is drafted in accordance with the provisions given in GB/ T 1.1-2009. This standard is the revision of QB/T 2881-2007 ?€?Antibacterial Technical Requirements for Footwear Linings and Insoles?€?. Compared with QB/T 2881-2007, the main technical changes are as follows.
?€? Change the standard name from ?€?Antibacterial Technical Requirements For Footwear Linings and Insoles?€? to ?€?Footwear and Footwear Components - Antibacterial Performance Specifications?€?;
?€? Expand the standard scope;
?€? Add and change the normative application documents;
?€? Add the terms and definitions of antibacterial shoes;
?€? Add the requirements for product identification;
?€? Add the sampling requirements;
?€? Modify the antibacterial test strains.
?€? Add the dissolution method and oscillation method. They are respectively as annex A and annex D;
?€? Add the dissolution indicators in requirements;
?€? Add the requirements for antibacterial agent in selection, which shall not affect the environment after product disposal;
?€? Adjust the indicators in table 1 of previous standard;
?€? Add Chapter 9 ?€?Judgment?€?.
This standard was proposed by China National Light Industry Council.
This standard shall be under the jurisdiction of National Technical Committee on Footwear of Standardization Administration of China (SAC/TC 305).
Drafting organizations of this standard. ANTA (CHINA) CO., LTD., China Leather and Footwear Industry Research Institute, Fujian Nan'an Bangdeng Shoes Industry Co., Ltd., Fujian Zhangping Yingchuan Light Industrial Co., Ltd., and Jinda Nano Tech (Xiamen) Co., Ltd.
Main drafters of this standard. Li Su, Zhang Weijuan, Hou Jingguo, Chen Junyuan, Wu Footwear and footwear components ?€?
Antibacterial performance Specifications
Warning. The test methods specified in this document require the use of related microorganisms. Only professionals who receive the training on microbiology and have practical experience can use microbiological testing method of this standard in laboratory with microbial treatment specifications. In consideration of laws and regulations of specific countries, appropriate safety measures shall be taken. 1 Scope
This standard specifies the terms and definitions, test environment, sampling, test strains, test method, requirements, determination, identification, and report of antibacterial performances of footwear and footwear components.
This standard applies to footwear and footwear components with antibacterial performance.
2 Normative??references
The articles contained in the following documents have become part od this document when they are quoted herein. For the dated documents so quoted, all the modifications (Including all corrections) or revisions made thereafter shall be applicable to this document.
GB 4789.2 National food safety standard Food microbiological examination. Aerobic plate count
GB/T 8629-2001 Textiles - Domestic washing and drying procedures for textile testing GB 19489 Laboratories - General requirements for biosafety
3 Terms??and??definitions
For the purpose of this standard, the following terms and definitions apply. 3.1
Dissolution Test Method (Inhibition-Zone Test)
A.1 Principle
Use antibacterial agent to dissolve out constantly, and form different concentration gradients through agar diffusion, so as to show bacteriostatic action. The test is judged by whether antibacterial agent dissolves out or conforms to dissolution requirements of this standard based on inhibition-zone size.
A 2 Test conditions
A.2.1 Main equipment
Constant temperature incubator (37??1)??C; freezer 5??C~10??C; second-level biological safety cabinet; pressure steam sterilizer; electrically heated drying oven; Vernier calliper; sterile test tubes; sterile pipette; sterile Erlenmeyer flask; inoculating loop; and alcohol lamp.
A.2.2 Preparation of materials
A.2.2.1 Control samples
It shall be the sterile dry filter paper in round of which the diameter is 15 mm, or in square of which the side length is 15 mm.
A.2.2.2 Samples
It shall be the suitable test samples in round of which the diameter is 15 mm, or in square of which the side length; the thickness is less than 4mm; cut from the antibacterial parts. A 2.2.3 Sterilization
Sterilize the test sample and control sample before testing. Samples that are not suitable for treatment with disinfectant may be dried directly after being rinsed with sterile water and receiving ultraviolet irradiation for 1 h. Other instruments used in test may be sterilized through hot and humid method OR hot and dry method.
A.2.3 Culture medium and reagents
A.2.3.1 Nutrient broth (NB)
Add 5.0 g of beef extract, 10.0 g of peptone, and 5.0 g of sodium chloride to 1000 mL of distilled water. After dissolution by heating, adjust the pH through 0.1 mol/L sodium Take 0.3 mL of bacterial solution that is prepared based on A.3.3; apply evenly to culture medium; cover with plate; stand for 5 min.
A.4.2 Sample placing
Paste to 3 contamination plates; each plate is pasted with 2 test samples and 2 control samples. Use sterile forceps to paste test sample to plate surface; sample center distance is less than 25 mm, and the distance from plate edge is not less than 15 mm. After pasting in place, use sterile forceps to gently press the samples so that the samples are very close to surface of the plate.
A.4.3 Culture and measurement
Cover the dish and cultivate at (37??1)??C for 16 h~18 h. Use Vernier calliper to measure inhibition-zone (including sample) and record. Note that growth of some microbes may restore and make bacteriostasis circle smaller if incubation time is too long. Full transparent inhibition-zone (the widest place in normal direction) shall be selected for inhibition-zone measurement. Measurement diameter shall be bounded by outer edge of inhibition-zone.
A.5 Expression of test results
A.5.1 Control sample shall not generate inhibition-zone, otherwise the test is invalid. A.5.2 Calculate the inhibition-zone width (D) based on formula (A.1); take arithmetic mean value of 3 samples, and retain 2 significant figures.
D ?€? Bacteriostatic ring width, in millimeters (mm)???
T ?€? Total width of bacteriostatic ring outer edge, in millimeter (mm);
R ?€? Total width of test sample, in millimeter (mm).
B.2.2.3 Sterilization
High-pressure steam sterilization is usually adopted at 115??C for 30 min. For the test samples that are not heat-resisting or that hot and humid disinfection method may easily affect antibacterial performance, other suitable methods may also be used for sterilization. Sterilization treatment before sample test shall not affect antibacterial performance of test sample.
B.2.3 Medium and reagents
B.2.3.1 Nutrient broth culture medium (NB)
See A.2.3.1.
B.2.3.2 Nutrient agar culture medium (NA)
See A.2.3.2.
B.2.3.3 Potato Dextrose Agar (PDA)
Add 200.0 g of peeled and diced potatoes; add 1000 mL of distilled water; boil for 10 min~20 min; filter with gauze; add additional distilled water to 1 000mL. Then add 20.0 g of glucose and 20.0 g of agar; after heating to dissolve and packing, place into pressure steam sterilizer at 115??C for 30 min.
B.2.3.4 Plate count agar (PCA)
Add 2.5 g of yeast extract, 5.0 g of tryptone, 1.0 g of glucose, and 15.0 g of agar to 1000 mL of distilled water or deionized water; place into a conical flask to mix...

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