QB/T 2738-2023 English PDF (QBT2738-2023)

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QB/T 2738-2023: Evaluating methods for antibacterial and bacteriostatic efficacy of daily chemical products
QB/T 2738-2023
QB
LIGHT INDUSTRY STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 71.100.40
CCS Y 40
Replacing QB/T 2738-2012
Evaluating methods for antibacterial and bacteriostatic
efficacy of daily chemical products
ISSUED ON: APRIL 21, 2023
IMPLEMENTED ON: NOVEMBER 01, 2023
Issued by: Ministry of Industry and Information Technology of PRC
Table of Contents
Foreword ... 3
1 Scope ... 5
2 Normative references ... 5
3 Terms and definitions ... 5
4 Basic requirements for laboratories and aseptic operations for testing the effects of
antibacterial and bacteriostatic daily chemical products ... 7
5 Sample collection ... 8
6 Principles for evaluating the antibacterial and bacteriostatic efficacy of daily chemical
products ... 8
7 Test methods for antibacterial and bacteriostatic efficacy of daily chemical products
... 9
8 Stability test method for antibacterial and bacteriostatic efficacy of daily chemical
products ... 38
Appendix A (Informative) Statistical test examples ... 39
References ... 42
Evaluating methods for antibacterial and bacteriostatic
efficacy of daily chemical products
1 Scope
This document describes the detection method of the antibacterial and bacteriostatic
efficacy of daily chemical products with special hygiene functions; stipulates the
evaluation criteria.
This document is applicable to the testing and evaluation of the antibacterial and
bacteriostatic properties of common detergents. The testing and evaluation of the
antibacterial and bacteriostatic properties of other daily chemical products are selected
according to the purpose.
2 Normative references
The contents of the following documents constitute the essential terms of this document
through normative references in the text. Among them, for dated references, only the
version corresponding to that date is applicable to this document; for undated references,
the latest version (including all amendments) is applicable to this document.
GB 4789.2 National food safety standard - Microbiological examination of food:
Aerobic plate count
QB/T 2153 Industrial oleic acid
QB/T 2850 General technical requirements for antibacterial and bacteriostatic
detergents
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Antibacterial
The process of killing bacteria or hindering bacterial growth and activity by
chemical or physical methods.
4 Basic requirements for laboratories and aseptic operations
for testing the effects of antibacterial and bacteriostatic daily
chemical products
4.1 Microbiological laboratories shall adopt a closed layout; the building shall be easy
to clean and disinfect. To avoid contamination, the test shall be carried out under
relatively positive pressure clean conditions. When pathogenic bacteria are used as
indicator bacteria due to special needs, they shall be carried out in a biological safety
cabinet (negative pressure).
4.2 Before the start of the test, the table and the indoor floor shall be cleaned by wet
methods; then the air in the laboratory shall be disinfected by ultraviolet rays or other
methods.
4.3 Experimenters shall wear work clothes, masks, hats; when conducting sterility tests,
they shall enter the laboratory after air showering. Then, wear sterile isolation clothes,
hats, masks correctly.
4.4 The sterile pipette shall be replaced every time a different sample is drawn; the
inoculation loop (needle) shall be burned and sterilized on the flame before it can be
used again.
4.5 Unless otherwise specified, only reagents and distilled water or deionized water or
water of equivalent purity, that are confirmed to be analytically pure, shall be used in
the analysis.
4.6 Reagents that require sterility, such as distilled water, phosphate buffer, culture
medium, bovine serum albumin, standard hard water, neutralizer, etc., shall be sterilized
or filtered.
4.7 Before using sterile equipment and reagents, check whether the container or
packaging is intact. If damaged, it shall not be used.
4.8 Sterile equipment and reagents in use shall not be exposed to the air for a long time.
4.9 When pipetting or inoculating, the mouth of the test tube and the agar plate shall be
close to the flame to prevent contamination.
4.10 All used contaminated equipment shall be immediately placed in a container
containing disinfectant, to prevent contamination of the surrounding environment and
clean items.
4.11 If the microbial culture is accidentally broken or other experimental
non-oxidizing fungicides);
c) 1.36 g potassium dihydrogen phosphate, 2.83 g disodium hydrogen phosphate,
3.0 g lecithin, 20.0 g Tween (80), 1000 mL distilled water (for non-oxidizing
fungicides);
d) 20.0 g Tween (80), 10.0 g sodium thiosulfate, 1000 mL PBS (for oxygen-type
fungicides).
7.2.2.5 Preparation of bacterial suspension
7.2.2.5.1 Preparation of bacterial propagule suspension
The preparation steps of bacterial propagule suspension are as follows.
a) Take the freeze-dried bacterial tube; open it under sterile operation; add an
appropriate amount of nutrient broth; gently blow and aspirate several times to
melt and disperse the bacterial strain. Take a test tube containing 5.0 mL ~ 10.0
mL of nutrient broth medium; add dropwise a small amount of bacterial
suspension; culture it at (36 ± 1) °C for 18 h ~ 24 h. Use an inoculation loop to
take the bacterial suspension of the first generation culture; streak it on the
nutrient agar medium plate; culture it at (36 ± 1) °C for 18 h ~ 24 h. Pick the
typical colony in the second generation culture above; inoculate it on the nutrient
agar slant; culture it at (36 ± 1) °C for 18 h ~ 24 h, which is the third generation
culture.
b) Take the fresh culture of the 3rd ~ 6th generation of the nutrient agar medium
slant (18 h ~ 24 h); use a 5.0 mL pipette to draw 3.0 mL ~ 5.0 mL of diluent into
the slant test tube; blow and suck repeatedly to wash off the bacterial moss. Then,
use a 5.0 mL pipette to transfer the washing liquid to another sterile test tube; mix
(oscillate) with an electric mixer for 20 seconds or tap on the palm 80 times, to
make the bacteria evenly suspended.
c) The preliminary bacterial suspension shall first be roughly measured by bacterial
concentration turbidimetric determination; then diluted with diluent to the
required concentration.
d) The bacterial propagule suspension shall be stored in a 4 °C refrigerator for future
use; it shall not be kept overnight for use on the same day.
e) When contamination is suspected, it shall be identified by colony morphology,
Gram staining, biochemical tests.
7.2.2.5.2 Preparation of Candida albicans suspension
The preparation steps of Candida albicans suspension are as follows:
a) Take a freeze-dried bacterial tube; open it with aseptic operation; use a capillary
pipette to add an appropriate amount of Sandcastle liquid culture medium into the
tube; gently blow and aspirate several times to melt and disperse the bacterial
strain. Take a test tube containing 5.0 mL ~ 10.0 mL of Sandcastle liquid culture
medium; add dropwise a small amount of bacterial suspension into it; culture it
at (36 ± 1) °C for 18 h ~ 24 h. Use an inoculation loop to take the bacterial
suspension of the first generation culture; streak it on the Sandcastle agar medium
plate; culture it at (36 ± 1) °C for 18 h ~ 24 h. Pick out the typical colonies in the
above second generati...