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NY/T 1331-2007 English PDF (NYT1331-2007)

NY/T 1331-2007 English PDF (NYT1331-2007)

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NY/T 1331-2007: Enumeration of Colony of Psychrotrophic Microorganisms, Total Aerobic Bacterial Spores and Thermophilic Aerobic Bacterial Spores in Milk and Dairy Products

Part I of NYT 1331-2007: specifies the test method for the enumeration of colony of psychrotrophic microorganisms in milk. This Part is applicable to the enumeration of colony of psychrotrophic microorganisms in raw and fresh milk and pasteurized milk. Part II of NYT 1331-2007: specifies the test method for enumeration of colony of aerobic bacterial spores in milk and dairy products. This Part is applicable to the enumeration of colony of aerobic bacterial spores in raw and fresh milk and its products. Part III of NYT 1331-2007: specifies the method for enumeration of colony of thermophilic aerobic bacterial spores in milk and dairy products. This Part is applicable to the enumeration of colony of thermophilic aerobic bacterial spores in raw and fresh milk and its products.
NY/T 1331-2007
ICS 67.100.10
X 16
PROFESSIONAL STANDARD
OF THE PEOPLE REPUBLIC OF CHINA
Enumeration of Colony of Psychrotrophic
Microorganisms, Total Aerobic Bacterial
Spores and Thermophilic Aerobic Bacterial
Spores in Milk and Dairy Products
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(ISO 6730. 1992, NEN 6813. 1999, NEN 6809. 1999, NEQ)
ISSUED ON. APRIL 17, 2007
IMPLEMENTED ON. JULY 01, 2007
Issued by. The Ministry of Agriculture of the People's Republic of
China
Foreword
This Standard is drafted by reference to "Milk - Enumeration of Colony-forming Units of Psychrotrophic Micro-organisms - Colony-Count Technique at 6.5???" (ISO 6730. 1992, English version), "Milk and Dairy Products-Determination of the Content of Spores of Mesophilic Aerobic Sporeforming Bacteria" (NEN 6813. 1999, Dutch version) and "Milk and Dairy Products - Determination of the Content of Thermophilic Aerobic Sporeforming Bacteria" (NEN 6809. 1999, Dutch version). This Standard is not equivalent to ISO 6730. 1992, NEN 6813. 1999, NEN 6809. 1999.
This Standard was proposed by the Ministry of Agriculture of the People's Republic of China.
This Standard is under the jurisdiction of Animal Husbandry of Standardization Administration of China.
Drafting organization of this Standard. Ministry of Agriculture Dairy Quality Supervision and Testing Center
Chief drafting staffs of this Standard. Li Qiying, Ma Wenhong, Zhang Hua and Wang Jinhua.
Table of Contents
Part I. Enumeration of Colony of Psychrotrophic Microorganisms in Milk ... 4 1 Scope ... 4
2 Normative References ... 4
3 Terms and Definitions ... 4
4 Principle ... 4
5 Diluent and Culture Medium ... 4
6 Equipment and Materials ... 5
7 Sampling ... 5
8 Test Procedures ... 5
9 Operational Steps ... 6
10 Result Calculation ... 7
11 Repeatability ... 7
Part II. Enumeration of Colony of Aerobic Bacterial Spores ... 9
1 Scope ... 9
2 Normative References ... 9
3 Terms and Definitions ... 9
4 Principle ... 9
5 Diluent and Culture Medium ... 9
6 Equipment and Materials ... 10
7 Sampling ... 10
8 Test Steps ... 10
9 Operation Procedures ... 11
10 Result Calculation ... 12
Part III. Enumeration of Colony of Thermophilic Aerobic Bacterial Spores ... 14 1 Scope ... 14
2 Normative References ... 14
3 Terms and Definitions ... 14
4 Principle ... 14
5 Diluent and Culture Medium ... 14
6 Equipment and Materials ... 15
7 Sampling ... 15
8 Test Procedures ... 15
9 Operational Steps ... 16
10 Result Calculation ... 17
References and Original Chinese Documents ... 19
Enumeration of Colony of Psychrotrophic Microorganisms,
Total Aerobic Bacterial Spores and Thermophilic Aerobic
Bacterial Spores in Milk and Dairy Products
Part I. Enumeration of Colony of Psychrotrophic Microorganisms
in Milk
1 Scope
This Part specifies the test method for the enumeration of colony of psychrotrophic microorganisms in milk.
This Part is applicable to the enumeration of colony of psychrotrophic microorganisms in raw and fresh milk and pasteurized milk.
2 Normative References
The following normative documents contain provisions which, through reference by this Part, constitute the provisions of this Part. For dated reference, subsequent amendments (excluding the corrected contents) or revisions do not apply to this Part. However, parties who enter into agreements based on this Standard are encouraged to investigate the possibility of applying the most recent editions of the relevant standards indicated below. For any undated references, the latest edition of the document applies to this Part.
GB/T 4789.1 Microbiological Examination of Food Hygiene - General Principles GB/T 4789.28-2003 Microbiological Examination of Food Hygiene - Staining Methods, Culture Mediums and Reagents
3 Terms and Definitions
The following terms and definitions are applicable to this Part.
3.1 Psychrotrophic microorganisms
Under condition of 6.5???, with aerobic incubation of 10d, the bacteria, yeasts and mildews forming countable colonies on MPC agar plate.
3.2 MPC agar
Milk plate count agar
4 Principle
Incubate the sample in the milk plate count agar, at 6.5??? for 10d, then count the number of psychrotrophic microorganisms per milliliter through colony counting. 5 Diluent and Culture Medium
5.1 Peptone salt solution
5.1.1 Components
Enzyme hydrolyzed casein peptone 1.0g
Sodium chloride 8.5g
Distilled water 1000mL
5.1.2 Preparation Method
shall not be greater than 30??? of the lower result; with the premise of - situations with greater-than-30??? of the lower result shall not exceed 5%.
Part II. Enumeration of Colony of Aerobic Bacterial Spores
1 Scope
This Part specifies the test method for enumeration of colony of aerobic bacterial spores in milk and dairy products.
This Part is applicable to the enumeration of colony of aerobic bacterial spores in raw and fresh milk and its products.
2 Normative References
The following normative documents contain provisions which, through reference by this Part, constitute the provisions of this Part. For dated reference, subsequent amendments (excluding the corrected contents) or revisions do not apply to this Part. However, parties who enter into agreements based on this Standard are encouraged to investigate the possibility of applying the most recent editions of the relevant standards indicated below. For any undated references, the latest edition of the document applies to this Part.
GB/T 4789.1 Microbiological Examination of Food Hygiene - General Principles GB/T 4789.28 - 2003 Microbiological Examination of Food Hygiene - Staining Methods, Culture Mediums and Reagents
3 Terms and Definitions
The following terms and definitions are applicable to this Part.
3.1 Total aerobic bacterial spores
Under aerobic condition, after 80??? heat treatment for 10min, the number of CFU of microorganisms on MPC agar plate with incubation of 48h at 36???.
3.2 Milk plate count agar
4 Principle
Take a certain amount of liquid sample or the primary diluent of sample. Heat it at 80??? for 10min. Incubate it in the milk plate count agar at 36??? for 48h. Calculate the total aerobic bacterial spores...

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