NY/T 1154.7-2006 English PDF (NYT1154.7-2006)

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NY/T 1154.7-2006: Pesticides guidelines for laboratory bioactivity tests. Part 7: Synergism evaluation of insecticide mixtures
NY/T 1154.7-2006
NY
AGRICULTURAL INDUSTRY STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
ICS 65.100
B 17
Pesticides guidelines for laboratory bioactivity tests -
Part 7: Synergism evaluation of insecticide mixtures
ISSUED ON: JULY 10, 2006
IMPLEMENTED ON: OCTOBER 01, 2006
Issued by: Ministry of Agriculture of PRC
Table of Contents
Foreword ... 3 
1 Scope ... 4 
2 Test conditions ... 4 
3 Test design ... 5 
4 Investigation ... 5 
5 Data statistics and analysis ... 6 
6 Results ... 7 
Pesticides guidelines for laboratory bioactivity tests -
Part 7: Synergism evaluation of insecticide mixtures
1 Scope
This part specifies the basic requirements and methods for the synergism
evaluation of insecticide mixtures of pesticides.
This part is applicable to the pesticide registration indoor test for the
determination of the combined action of pesticides. Other tests shall be carried
out with reference to this part.
2 Test conditions
2.1 Test target
The test target shall be a sensitive test insect raised indoors, indicating the
source of the test insect and the breeding conditions.
2.2 Equipment
2.2.1 Electronic balance (sensitivity 0.1 mg);
2.2.2 Filter paper;
2.2.3 Microdropper;
2.2.4 Tissue culture plate;
2.2.5 Potter spray tower;
2.2.6 Triangular bottle;
2.2.7 Writing brush;
2.2.8 Culture dish;
2.2.9 Beaker;
2.2.10 Pipette, etc.
3 Test design
3.1 Preparation of test materials
Choose sensitive test insects raised indoors and consistent in age. If the insect
is collected from the field, the test shall be carried out after 1 generation ~ 2
generations are raised indoors. When the insect is large (such as cotton
bollworm, cabbage caterpillar, etc.), the number of test insects per dose is not
less than 40; when the insect is small (such as aphids, red spiders, etc.), the
number of test insects per dose is not less than 120.
3.2 Drug
The test drug shall be the original drug (parent drug), indicating the generic
name, trade name or code, content, manufacturer.
3.3 Test procedure
3.3.1 Preparation of liquid drug
Water-soluble drugs are directly dissolved and diluted by water. Other drugs
shall be dissolved in suitable organic solvents (such as acetone, chloroform,
ethanol, etc.); then diluted by the aqueous solution containing a suitable amount
of surfactant such as 0.1% Tween 80 (or 0.1% Triton-X100). Prepare a single-
dose parent liquid separately. Design more than 5 groups of ratios according to
the mixing purpose and the activity of the agent. Each single-dose and each
group of mixing agents are set to 5 ~ 7 series of mass concentrations according
to the equal ratio or equal difference method.
3.3.2 Drug treatment
Select the corresponding test methods, such as the drug film method, drip
method, spray method, leaf poisoning method, dipping method, etc., according
to the specific requirements of insect species, drug action characteristics, action
mode, the test itself. Each treatment is repeated 4 times. The corresponding
organic solvent treated which containing no agent is set as a control.
4 Investigation
Investigate the death of test insects 24 hours after treatment. Record the total
number of insects and the number of dead insects. The investigation time can
be shortened or extended according to the test requirements and the
characteristics of the drug.