GB/T 7297-2006 English PDF (GBT7297-2006)

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GB/T 7297-2006: Feed additive -- Vitamin B2 (riboflavin)
GB/T 7297-2006
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 65.120
B 46
Replacing GB 7297-1987
Food additive - Vitamin B2 (riboflavin)
饲料添加剂 维生素 B2(核黄素)
ISSUED ON: DECEMBER 20, 2006
IMPLEMENTED ON: MARCH 01, 2007
Issued by: General Administration of Quality Supervision, Inspection and
Quarantine of PRC;
Standardization Administration of PRC.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Requirements ... 4
4 Test method ... 5
5 Inspection rules ... 8
6 Labeling, packaging, transportation, storage ... 9
Food additive - Vitamin B2 (riboflavin)
1 Scope
This standard specifies the technical requirements, test methods, inspection rules,
labeling, packaging, transportation, storage of feed additive vitamin B2 (riboflavin)
products.
This standard applies to vitamin B2, which is produced by biological fermentation or
chemical synthesis, used as vitamin feed additives in the feed industry.
Molecular formula: C17H20N4O6
Relative molecular mass: 376.37 (international relative atomic mass in 1999)
2 Normative references
The provisions in following documents become the provisions of this Standard through
reference in this Standard. For the dated references, the subsequent amendments
(excluding corrections) or revisions do not apply to this Standard; however, parties who
reach an agreement based on this Standard are encouraged to study if the latest versions
of these documents are applicable. For undated references, the latest edition of the
referenced document applies.
GB/T 6435 Determination of moisture and other volatile mater content in feeds
GB/T 6682 Water for analytical laboratory use - Specification and test methods
GB 10648 Feed label
GB/T 13080 Determination of lead in feeds - Method using atomic absorption
spectrometry
GB/T 14699.1 Feeding Stuffs - Sampling
Pharmacopoeia of the People's Republic of China (2005 edition)
3 Requirements
3.1 Specifications
96%, 98%.
4.1.9 Sulfuric acid.
4.2 Instruments and equipment
4.2.1 Common laboratory equipment.
4.2.2 UV spectrophotometer, which is equipped with a 1 cm cuvette.
4.2.3 Polarimeter.
4.2.4 Atomic absorption spectrophotometer.
4.3 Identification test
4.3.1 Weigh about 1 mg of the sample. Add 100 mL of water to dissolve it. The solution
will appear yellow-green and have strong yellow-green fluorescence, under transmitted
light. Divide it into 2 parts. Add inorganic acid or alkali solution to 1 part; the
fluorescence will be disappeared. Add a little sodium dithionite crystal to the other part.
After shaking well, the yellow color disappears and the fluorescence disappears.
4.3.2 Determine the prepared solution according to the content. Use a
spectrophotometer to make determination. Use a 1 cm cuvette, to measure the
absorption spectrum of the specimen solution, within the wavelength range of 200 nm
to 500 nm. It shall have maximum absorption, at the wavelength of 267 nm ± 1 nm, 375
nm ± 1 nm, 444 nm ± 1 nm.
The ratio -- of the absorbance at 375 nm to the absorbance at 267 nm -- shall be 0.31 ~
0.33. The ratio -- of the absorbance at 444 nm to the absorbance at 267 nm -- shall be
0.36 ~ 0.39.
4.4 Determination of vitamin B2 content
4.4.1 Principle
After the vitamin B2 in the specimen is dissolved by alkali, its concentration in the test
solution is directly proportional to the ultraviolet absorption value at 444 nm
wavelength; its percentage content is determined accordingly.
4.4.2 Analytical procedures
Note: Operate in dark!
Weigh about 0.065 g of the specimen (accurate to 0.0002 g). Put it in a 500 mL brown
volumetric flask. Add 5 mL of water, to make the sample completely wet. Add 5 mL of
sodium hydroxide solution (4.1.2), to dissolve it completely. Immediately add 100 mL
of water and 2.5 mL of glacial acetic acid (4.1.4). Add water to dilute it to the mark.
Shake well. Accurately pipette 10 mL of test solution, into a 100 mL brown volumetric
flask. Add 1.8 mL of sodium acetate solution (4.1.5). Use water to dilute it to the mark.
Weigh 1 g ~ 2 g of the sample (accurate to 0.01 g). Place it in a porcelain crucible, that
has been burned at 700 °C ~ 800 °C to a constant weight. Heat slowly over a low fire,
until it is completely carbonized. Let it cool. Add 0.5 mL ~ 1 mL of sulfuric acid (4.1.9)
to make it moist. Heat at low temperature, until the sulfuric acid vapor is completely
removed. Transfer it to a muffle furnace. Burn it at 700 °C ~ 800 °C, until it reaches a
constant volume.
4.8.2 Calculations and presentation of results
Residue on ignition X2 (expressed in mass fraction, value in %) is calculated according
to formula (2):
Where:
m1 - The mass of the crucible plus residue, in grams (g);
m2 - The crucible mass, in grams (g);
m - The sample mass, in grams (g).
4.8.3 Repeatability
Results retain two significant figures.
The relative deviation of the results of two simultaneous parallel determinations of the
same sample, by the same analyst, shall not be greater than 5%.
4.9 Determination of lead
It is measured according to GB/T 13080.
4.10 Determination of arsenic
Accurately weigh 1 g of the specimen (accurate to 0.0002 g). Make measurement,
according to the first method for the determination of arsenic in the Pharmacopoeia of
the People's Republic of China (2005 edition) (Gule's method).
5 Inspection rules
5.1 The manufacturer shall ensure that all products meet the requirements specified in
this standard. Each batch of products shall be accompanied by a product certificate.
Items stipulated in this standard are exit-factory inspection items, except arsenic and
lead.
5.2 Type inspection: In one of the following situations, the quality of feed additive
vitamin B2 shall be type inspected. The inspection items include all items specified in
this standard:
a) After formal production, when raw materials and processes are changed;
b) After formal production, a type inspection shall be carried out every six months;
c) When production is stopped and then resumed, type inspection shall be carried
out;
d) When the product quality supervision department makes a request for type
inspection.
5.3 The user organization has the right to inspect and accept the received vitamin B2
products, in accordance with the provisions of this standard. The acceptance time is
within one month of the arrival of the goods.
5.4 Sampling method: It is carried out, according to the provisions of GB/T 14699.1.
Clean, dry, airtight, light-proof sample bottles shall be prepared for sampling. The
bottles shall be labeled with the manufacturer, product name, batch number, sampling
date. When sampling, use a clean and applicable sampling tool, to insert three-quarters
of the depth of the material layer. Fully mix the sample taken. Divide it into quarters.
Each batch of samples is divided into two parts. The amount of each part of sample
shall be 3 times the required specimen amount for inspection. Contain it into sample
bottles: one bottle is used for inspection; the other bottle is sealed for future reference.
5.5 Judgment rules: If one indicator of the inspection result does not meet the
requirements of this standard, it shall double the samples for re-inspection. If only one
indicator of the re-inspection result does not meet the requirements of this standard, the
entire batch of products will be judged as unqualified.
5.6 Arbitration: If ...