GB/T 45235-2025 English PDF (GB/T45235-2025)

GB/T 45235-2025: Determination of bisphenol A in electrical and electronic products - High performance liquid chromatography
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GB/T 45235-2025
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 31.020
CCS L 10
Determination of Bisphenol A in Electrical and Electronic
Products – High Performance Liquid Chromatography
ISSUED ON. JANUARY 24, 2025
IMPLEMENTED ON. AUGUST 1, 2025
Issued by. State Administration for Market Regulation;
Standardization Administration of the People’s Republic of China.
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative References... 4
3 Terms and Definitions... 4
4 Principle... 5
5 Reagents and Materials... 5
6 Instrumentation... 6
7 Preparation of Sample... 6
8 Test Procedures... 6
9 Calculation of Results... 8
10 Limit of Quantification and Precision... 8
11 Test Report... 9
Appendix A (Informative) Liquid Chromatogram of BPA Standard Working Solution
... 10
Determination of Bisphenol A in Electrical and Electronic
Products – High Performance Liquid Chromatography
WARNING. People who use this Document shall have practical working experience in
regular laboratory. This Document does not point out all possible safety issues. Users are
responsible for taking appropriate safety and health measures and ensuring that the
conditions stipulated by relevant national laws and regulations are met.
1 Scope
This Document describes the principle, reagents and materials, instrumentation, preparation of
sample, test procedures, calculation of results, Limit of quantification and precision, and test
report for the determination of bisphenol A content in electronic and electrical products by high
performance liquid chromatography.
This Document is applicable to the determination of bisphenol A content in polymer materials
of electronic and electrical products.
2 Normative References
The provisions in following documents become the essential provisions of this Document
through reference in this Document. For the dated documents, only the versions with the dates
indicated are applicable to this Document; for the undated documents, only the latest version
(including all the amendments) is applicable to this Document.
GB/T 6682 Water for analytical laboratory use - Specification and test methods
GB/T 39560.1 Determination of certain substances in electrical and electronic products -
Part 1.Introduction and overview
GB/T 39560.2 Determination of certain substances in electrical and electronic products -
Part 2.Disassembly, disjointment and mechanical sample preparation
3 Terms and Definitions
For the purposes of this Document, the terms and definitions given in GB/T 39560.1 and the
following apply.
3.1 Bisphenol A; BPA
5.9 Centrifuge tube. 10 mL, with cap.
5.10 Glass vial. 50 mL, with sealing cap.
5.11 Syringe. 2 mL.
5.12 Liquid chromatography sample bottle. About 1.5 mL, with cap.
5.13 Sealing film.
6 Instrumentation
6.1 High performance liquid chromatograph. Equipped with fluorescence detector.
6.2 Electronic balance. With sensitivity of 0.1 mg.
6.3 Grinder. With 1 mm pore size filter screen.
6.4 Water bath ultrasonic generator, power per unit area ≥ 0.3 W/cm2.
6.5 Pipettor or pipette tube. 10 mL, 30 mL.
6.6 Rotary oscillator.
6.7 Centrifuge. Speed ≥ 5,000 r/min.
7 Preparation of Sample
Sampling is conducted according to GB/T 39560.2.Cut the sample into particles no less than 5
mm with scissors and crush it into small particles less than 1 mm with a grinder (6.3) as the
specimen to be tested. Liquid nitrogen shall be used to cool the specimen fully before grinding.
The cooling time should be no less than 15 min.
8 Test Procedures
8.1 Ultrasonic extraction
Weigh 1 g (accurate to 0.1 mg) of the crushed specimen in Clause 7 and carefully put it into the
bottom of the glass vial (5.10). Use a pipettor or pipette tube (6.5) to accurately transfer 10 mL
of tetrahydrofuran (5.2) and slowly add it along the inner wall of the glass vial containing the
specimen. Cover the bottle cap tightly and seal it with a sealing film (5.13). Place it in a water
bath ultrasonic generator (6.4) for ultrasonic oscillation for 60 min. After the ultrasonic
oscillation is completed, place it at room temperature to cool to room temperature.
8.2 Dilution
Open the cap of the glass vial in 8.1; use a pipettor or pipette tube (6.5) to accurately transfer
30 mL of methanol-water mixture (5.4); slowly titrate it into the opened glass vial; close the
cap. Place the glass vial in a rotary oscillator (6.6) and shake for about 0.5 min to fully mix the
extract in the glass vial.
8.3 Centrifugal separation
Open the cap of the glass vial in 8.2; use a pipettor or pipette tube (6.5) to transfer about 10 mL
of extract into a centrifuge tube (5.9); cover the cap and place it in a centrifuge (6.7); centrifuge
at 5,000 r/min for 5 min. Use a syringe (5.11) to take out about 2 mL of the supernatant in the
centrifuge tube; filter it through a membrane filter (5.8); and inject it into a liquid
chromatography sample bottle (5.12) for testing.
8.4 High-performance liquid chromatography determination
8.4.1 Instrument conditions
The test results depend on the used instrument, and it is impossible to give general parameters
for high-performance liquid chromatography (with fluorescence detector). The following
parameters have been proven to be feasible.
a) Liquid chromatography column. C18, 250 mm×4.6 mm (i.d.), particle size 5μm, or
equivalent performance.
b) Column temperature. 30 ℃.
c) Mobile phase. methanol-water (70+30, volume ratio).
d) Flow rate. 1 mL/min.
e) Injection volume. 10.0 μL.
f) Detector wavelength. Excitation wavelength, 275 nm; emission wavelength, 313 nm.
g) Run time. 15 min.
8.4.2 Standard working curve
The BPA standard intermediate solution (5.7) is prepared with methanol-water mixture (5.4) to
prepare BPA standard solutions of concentration series of 0.1 μg/mL, 0.2 μg/mL, 0.5 μg/mL,
1.0 μg/mL and 2.0 μg/mL, respectively. It shall be prepared for immediate use. The BPA
standard solutions of the above concentration series are measured according to the instrument
analysis conditions recommended in 8.4.1.The standard working curve is drawn with the
concentration of BPA as the horizontal axis and the corresponding chromatographic peak area
as the vertical axis, but not exceeding the origin. The linear correlation coefficient of the
standard working curve shall be no less than 0.995; otherwise, the BPA standard solutions of
each concentration series are re-prepared, and the standard working curve is re-tested and drawn.
GB/T 45235-2025
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 31.020
CCS L 10
Determination of Bisphenol A in Electrical and Electronic
Products – High Performance Liquid Chromatography
ISSUED ON. JANUARY 24, 2025
IMPLEMENTED ON. AUGUST 1, 2025
Issued by. State Administration for Market Regulation;
Standardization Administration of the People’s Republic of China.
Table of Contents
Foreword... 3
1 Scope... 4
2 Normative References... 4
3 Terms and Definitions... 4
4 Principle... 5
5 Reagents and Materials... 5
6 Instrumentation... 6
7 Preparation of Sample... 6
8 Test Procedures... 6
9 Calculation of Results... 8
10 Limit of Quantification and Precision... 8
11 Test Report... 9
Appendix A (Informative) Liquid Chromatogram of BPA Standard Working Solution
... 10
Determination of Bisphenol A in Electrical and Electronic
Products – High Performance Liquid Chromatography
WARNING. People who use this Document shall have practical working experience in
regular laboratory. This Document does not point out all possible safety issues. Users are
responsible for taking appropriate safety and health measures and ensuring that the
conditions stipulated by relevant national laws and regulations are met.
1 Scope
This Document describes the principle, reagents and materials, instrumentation, preparation of
sample, test procedures, calculation of results, Limit of quantification and precision, and test
report for the determination of bisphenol A content in electronic and electrical products by high
performance liquid chromatography.
This Document is applicable to the determination of bisphenol A content in polymer materials
of electronic and electrical products.
2 Normative References
The provisions in following documents become the essential provisions of this Document
through reference in this Document. For the dated documents, only the versions with the dates
indicated are applicable to this Document; for the undated documents, only the latest version
(including all the amendments) is applicable to this Document.
GB/T 6682 Water for analytical laboratory use - Specification and test methods
GB/T 39560.1 Determination of certain substances in electrical and electronic products -
Part 1.Introduction and overview
GB/T 39560.2 Determination of certain substances in electrical and electronic products -
Part 2.Disassembly, disjointment and mechanical sample preparation
3 Terms and Definitions
For the purposes of this Document, the terms and definitions given in GB/T 39560.1 and the
following apply.
3.1 Bisphenol A; BPA
5.9 Centrifuge tube. 10 mL, with cap.
5.10 Glass vial. 50 mL, with sealing cap.
5.11 Syringe. 2 mL.
5.12 Liquid chromatography sample bottle. About 1.5 mL, with cap.
5.13 Sealing film.
6 Instrumentation
6.1 High performance liquid chromatograph. Equipped with fluorescence detector.
6.2 Electronic balance. With sensitivity of 0.1 mg.
6.3 Grinder. With 1 mm pore size filter screen.
6.4 Water bath ultrasonic generator, power per unit area ≥ 0.3 W/cm2.
6.5 Pipettor or pipette tube. 10 mL, 30 mL.
6.6 Rotary oscillator.
6.7 Centrifuge. Speed ≥ 5,000 r/min.
7 Preparation of Sample
Sampling is conducted according to GB/T 39560.2.Cut the sample into particles no less than 5
mm with scissors and crush it into small particles less than 1 mm with a grinder (6.3) as the
specimen to be tested. Liquid nitrogen shall be used to cool the specimen fully before grinding.
The cooling time should be no less than 15 min.
8 Test Procedures
8.1 Ultrasonic extraction
Weigh 1 g (accurate to 0.1 mg) of the crushed specimen in Clause 7 and carefully put it into the
bottom of the glass vial (5.10). Use a pipettor or pipette tube (6.5) to accurately transfer 10 mL
of tetrahydrofuran (5.2) and slowly add it along the inner wall of the glass vial containing the
specimen. Cover the bottle cap tightly and seal it with a sealing film (5.13). Place it in a water
bath ultrasonic generator (6.4) for ultrasonic oscillation for 60 min. After the ultrasonic
oscillation is completed, place it at room temperature to cool to room temperature.
8.2 Dilution
Open the cap of the glass vial in 8.1; use a pipettor or pipette tube (6.5) to accurately transfer
30 mL of methanol-water mixture (5.4); slowly titrate it into the opened glass vial; close the
cap. Place the glass vial in a rotary oscillator (6.6) and shake for about 0.5 min to fully mix the
extract in the glass vial.
8.3 Centrifugal separation
Open the cap of the glass vial in 8.2; use a pipettor or pipette tube (6.5) to transfer about 10 mL
of extract into a centrifuge tube (5.9); cover the cap and place it in a centrifuge (6.7); centrifuge
at 5,000 r/min for 5 min. Use a syringe (5.11) to take out about 2 mL of the supernatant in the
centrifuge tube; filter it through a membrane filter (5.8); and inject it into a liquid
chromatography sample bottle (5.12) for testing.
8.4 High-performance liquid chromatography determination
8.4.1 Instrument conditions
The test results depend on the used instrument, and it is impossible to give general parameters
for high-performance liquid chromatography (with fluorescence detector). The following
parameters have been proven to be feasible.
a) Liquid chromatography column. C18, 250 mm×4.6 mm (i.d.), particle size 5μm, or
equivalent performance.
b) Column temperature. 30 ℃.
c) Mobile phase. methanol-water (70+30, volume ratio).
d) Flow rate. 1 mL/min.
e) Injection volume. 10.0 μL.
f) Detector wavelength. Excitation wavelength, 275 nm; emission wavelength, 313 nm.
g) Run time. 15 min.
8.4.2 Standard working curve
The BPA standard intermediate solution (5.7) is prepared with methanol-water mixture (5.4) to
prepare BPA standard solutions of concentration series of 0.1 μg/mL, 0.2 μg/mL, 0.5 μg/mL,
1.0 μg/mL and 2.0 μg/mL, respectively. It shall be prepared for immediate use. The BPA
standard solutions of the above concentration series are measured according to the instrument
analysis conditions recommended in 8.4.1.The standard working curve is drawn with the
concentration of BPA as the horizontal axis and the corresponding chromatographic peak area
as the vertical axis, but not exceeding the origin. The linear correlation coefficient of the
standard working curve shall be no less than 0.995; otherwise, the BPA standard solutions of
each concentration series are re-prepared, and the standard working curve is re-tested and drawn.