GB/T 35949-2018 English PDF (GBT35949-2018)

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GB/T 35949-2018: Determination of prohibited aristolochic acid A in cosmetics -- High performance liquid chromatography
GB/T 35949-2018
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 71.100.70
Y 42
Determination of prohibited aristolochic acid A in
cosmetics - High performance liquid chromatography
ISSUED ON. FEBRUARY 06, 2018
IMPLEMENTED ON. SEPTEMBER 01, 2018
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine;
Standardization Administration of the People's Republic of
China.
Table of Contents
Foreword ... 3 
Introduction ... 4 
1 Scope ... 5 
2 Normative references ... 5 
3 Principle of high performance liquid chromatography ... 5 
4 Reagents and materials ... 5 
5 Instruments and equipment... 6 
6 Analysis procedures ... 6 
7 Calculation of result ... 7 
8 Recovery and precision ... 8 
9 Allowable difference ... 8 
Annex A (informative) Chinese name, CAS number, molecular formula, relative
molecular mass, chemical structure of reference substance ... 9 
Annex B (informative) Liquid chromatogram of aristolochic acid A standard
solution ... 10 
Annex C (informative) Confirmation test ... 11 
Determination of prohibited aristolochic acid A in
cosmetics - High performance liquid chromatography
1 Scope
This Standard specifies reagents and materials, instruments and equipment,
analysis procedures, calculation of result, recovery and precision, allowable
difference, etc. for high performance liquid chromatography for prohibited
aristolochic acid A in cosmetics.
This Standard is applicable to the determination of aristolochic acid A in skin
care cosmetics such as creams, lotions, toning lotions.
The detection limit of this Standard method is 0.3mg/kg, and the limit of
quantification is 1.0mg/kg.
2 Normative references
The following referenced documents are indispensable for the application of
this document. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any
amendments) applies.
GB/T 6682, Water for analytical laboratory use - Specification and test
methods
3 Principle of high performance liquid chromatography
The sample is ultrasonically extracted and filtered with methanol and detected
by high performance liquid chromatograph equipped with a UV detector.
Quantify by external standard method.
4 Reagents and materials
Unless otherwise stated, all reagents are analytically pure, and the water is the
grade one water specified in GB/T 6682.
4.1 Methanol. chromatographically pure.
4.2 Glacial acetic acid.
6.3 Drawing of calibration curve
Respectively take 0.1mL, 0.5mL, 1.0mL, 2.0mL, 5.0mL of standard stock
solutions (4.4). Use grade one water to set volume to 100mL. Prepare the
standard working solutions of which the concentrations are 1.0μg/mL, 5.0μg/mL,
10.0μg/mL, 20.0μg/mL, 50.0μg/mL. Take standard working solutions at all
levels into the high performance liquid chromatograph. Draw a calibration curve
with peak area and solution concentration. For the liquid chromatogram of
aristolochic acid A standard solution, see Figure B.1 in Annex B.
6.4 Sample determination
Take the sample solution (6.1) into the high performance liquid chromatograph.
Determine the nature according to peak retention time and UV spectrum.
Record chromatographic peak area. Obtain the corresponding concentration of
aristolochic acid A from the calibration curve.
6.5 Qualitative confirmation
Conduct qualitative determination of sample by liquid chromatograph. When
performing sample determination, if the detected peak value of the aristolochic
acid A is consistent with the standard substance, and the sample chromatogram
after deducting the background, the UV absorption spectrum of the material is
consistent with the UV absorption spectrum of the reference material, it shall
initially confirm the presence of aristolochic acid A. If necessary, positive
samples shall be confirmed by liquid chromatography-mass spectrometry. See
Annex C for confirmation test.
6.6 Blank test
Except for not taking sample, follow the above steps.
6.7 Parallel test
According to the above steps, perform the parallel test determination of the
same sample.
7 Calculation of result
The content of aristolochic acid A in the sample is calculated by the
chromatographic data processor or according to formula (1).
where,
Annex C
(informative)
Confirmation test
C.1 Liquid chromatography reference conditions
Liquid chromatography reference conditions are as follows.
a) Chromatographic column. EclipsePlus C18 (150mm x 2.1mm, 1.8μm), or
equivalent column;
b) Mobile phase. 5mmol/L ammonium acetate solution (pH adjusted to 7.5
with ammonia water) - acetonitrile = 75-25 (volume ratio);
c) Flow rate. 0.2mL/min;
d) Column temperature. 30°C;
e) Injection volume. 5μL.
C.2 Mass spectrometry reference conditions
Mass spectrometry reference conditions are as follows.
a) Ion source. electrospray ion source (ESI);
b) Ionization mode. positive ion mode;
c) Drying gas temperature. 350°C;
d) Dry gas flow. 10L/min;
e) Sheath gas temperature. 300°C;
f) Sheath gas flow. 7L/min;
g) Capillary voltage. 4000V;
h) Data acquisition. multiple response monitoring (MRM) mode.
C.3 Qualitative determination
When the sample is tested, the sample solution is diluted appropriately. Perform
determination of sample solution and standard working solution by liquid
chromatography-mass spectrometry. If the selected ions are present and the