GB/T 18246-2019: Determination of amino acids in feeds

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Scope

This Standard specifies the determination methods for total amino acids (peptide
bonded and free) and free amino acids (natural and added) in feeds, including
conventional acid hydrolysis method, oxidative acid hydrolysis method, alkaline
hydrolysis method and acid extraction method.
The conventional acid hydrolysis method applies to the determination of the content of
sulfur-containing amino acids (cystine, cysteine, methionine) and other amino acids
other than tryptophan (aspartic acid, threonine, serine, glutamic acid, proline, glycine,
alanine, valine, isoleucine, leucine, tyrosine, phenylalanine, lysine, histidine, arginine)
in feed raw materials, compound feeds, concentrated feeds, concentrate supplements
and additive premixes.
The oxidative acid hydrolysis method applies to the determination of sulfur-containing
amino acids (cystine, cysteine and methionine) in feed raw materials, compound feeds,
concentrated feeds and concentrate supplements. When sodium metabisulfite is used as
the oxidation terminator, other proteolytic amino acids except tyrosine and tryptophan
can be determined; when hydrobromic acid is used as the terminator, other proteolytic
amino acids except tyrosine, phenylalanine, histidine and tryptophan can be determined.
The alkaline hydrolysis method applies to the determination of tryptophan in feed raw
materials, compound feeds, concentrated feeds and concentrate supplements.
The acid extraction method applies to the determination of free amino acids in
compound feeds, concentrated feeds, concentrate supplements and additive premixes.
The quantitative limits of each amino acid are shown in Table A.1 in Appendix A.

Basic Data

Standard ID	GB/T 18246-2019 (GB/T18246-2019)
Description (Translated English)	Determination of amino acids in feeds
Sector / Industry	National Standard (Recommended)
Classification of Chinese Standard	B46
Classification of International Standard	65.120
Word Count Estimation	14,195
Date of Issue	2019-12-10
Date of Implementation	2020-07-01
Older Standard (superseded by this standard)	GB/T 18246-2000
Quoted Standard	GB/T 6682; GB/T 15399; GB/T 15400; GB/T 20195; JJG 1064
Issuing agency(ies)	State Administration for Market Regulation, China National Standardization Administration
Summary	This standard specifies methods for the determination of total amino acids (peptide-bonded and free) and free amino acids (natural and added) in feed, including conventional acid hydrolysis, oxidative acid hydrolysis, alkaline hydrolysis and acid extraction. The conventional acid hydrolysis method is suitable for sulfur-containing amino acids (cystine, cysteine, methionine) and amino acids other than tryptophan (tianmen) in feed raw materials, compound feeds, concentrated feeds, concentrate supplements and additive premix feeds. Partic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, tyrosine, phenylalanine, lysine, Determination of histidine, arginine) content. The oxidative acid hydrolysis method is suitable for sulfur-containing amino acids (cystine, cysteine ??and egg yolk) in feed ingredients, compound feeds, concentrated feeds and concentrate supplements.