GB/T 18204.1-2000 English PDF (GB/T18204.1-2000)

GB/T 18204.1-2000: Methods of microbiological examination for air in public places - Determination of aerobic bacterial count
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GB/T 18204.1-2000
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
Methods of microbiological examination for air in
public places -
Determination of aerobic bacterial count
ISSUED ON. SEPTEMBER 30, 2000
IMPLEMENTED ON. JANUARY 01, 2001
Issued by. National Quality and Technical Supervision Bureau
GB
Tips - GB 18204 Series (not part of this Standard)
Standard Description Issued Date
Enforced
Date Newer Version (Click to Check)
GB/T 18204.1-2000 Methods of microbiological examination for air in public places - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.2-2000 Methods of microbiological examination for tea set in public places - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.3-2000 Methods of microbiological examination for tea set in public places - Determination of coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.4-2000 Methods of microbiological examination for towel and bedclothes in public places - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.5-2000 Methods of microbiological examination for towel and bedclothes in public places - Determination of Coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.6-2000 Methods of microbiological examination for barber's tools - Determination of Coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.7-2000 Methods of microbiological examination for barber's tools - Determination of Staphylococcus aureus 2000-09-30 2001-01-01
GB/T 18204.8-2000 Methods of microbiological examination for slippers in public places - Determination of molds and yeasts count 2000-09-30 2001-01-01
GB/T 18204.9-2000 Methods of microbiological examination for water in swimming pool - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.10-2000 Methods of microbiological examination for water in swimming pool - Determination of Coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.11-2000 Methods of microbiological examination for bath tube and wash basin in public places - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.12-2000 Methods of microbiological examination for bath tube and wash basin in public places - Determination of Coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.16-2000 Method for determination of atmospheric pressure in public places 2000-09-30 2001-01-01
GB/T 18204.18-2000 Method for determination of air change flow of indoor air in public places 2000-09-30 2001-01-01
GB/T 18204.19-2000 Method for measurement of infiltration rates of indoor air in public places 2000-09-30 2001-01-01
GB/T 18204.23-2000 Methods for determination of carbon monoxide in air of public places 2000-09-30 2001-01-01
GB/T 18204.28-2000 Method for determination of temperature in water of swimming place 2000-09-30 2001-01-01
GB/T 18204.1-2013 Hygiene inspection methods in public places - Part 1. Physical factors 2014-12-01
GB/T 18204.3-2013 Hygiene inspection methods in public places - Part 3. Airborne microbes 2014-12-01
GB/T 18204.4-2013 Hygiene inspection methods in public places - Part 4. Public appliances microorganisms 2014-12-01
GB/T 18204.5-2013 Hygiene inspection methods in public places - Part 5. Air conditioning system 2014-12-01
GB/T 18204.6-2013 Hygiene inspection methods in public places - Part 6. Health monitoring technical specifications 2014-12-01
Table of Contents
Foreword ... 6
1 Scope ... 7
2 Definitions... 7
3 Instruments and devices ... 7
4 Medium ... 8
5 Operational steps ... 8
Foreword
In order to implement the "Public Places Hygienic Management Regulations" and GB
9663~9673-1996, GB 16153-1996 "Public Places Hygienic Standard”, and to strengthen
the hygienic supervision and management of public places, this Standard is formulated.
The methods in this Standard are the supervision and inspection methods supporting to
GB 9663~9673-1996.
The first method of this Standard is the arbitration method.
This Standard is released for the first-time.
This Standard is proposed by Ministry of Health, the People's Republic of China.
Drafting organizations of this Standard. Chinese Academy of Preventive Medicine and
Environmental Hygiene Monitoring Institute, Beijing Municipal Health and Epidemic
Prevention Station, Guangdong Provincial Health and Epidemic Prevention Station, and
Tianjing Municipal Health and Epidemic Prevention Station.
The main drafters of this Standard. Zhou Shuyu, Chen Xiping, Gao Hui, Cai Han, and
Zhang Shulan.
Methods of microbiological examination for air in public places -
Determination of aerobic bacterial count
1 Scope
This Standard specifies the determination method of total number of bacteria in the air of
public places.
This Standard applies to the determination of total number of bacteria in the air of public
places.
2 Definitions
This Standard uses the following definitions.
2.1 Impacting method
It refers to the sampling determination method - USE percussive air microbial sampler to
sample; through pumping dynamic action, LET air go through slits or small holes so as to
generate high-speed airflow; so that the bacteria-carrying particles suspended in air hit
onto nutrient agar plate; after incubating at 37ºC for 48 h; CALCULATE the number of
bacterial colonies contained in each cubic meter of air.
2.2 Natural sinking method
It refers to the sampling determination method - A nutrient agar plate, diameter of 9 cm, is
exposed for 5 min at the sampling point; after incubating at 37ºC for 48 h; CALCULATE
the number of grown bacterial colonies.
3 Instruments and devices
3.1 High-pressure steam sterilizer.
3.2 Dry-heat Sterilizer.
3.3 Thermostat incubator.
3.4 Refrigerator.
3.5 Petri dish (diameter of 9 cm).
3.6 General devices for preparing medium. Measuring bottle, conical flask, pH meter or
precision pH paper, etc.
3.7 Percussive air microbial sampler.
and shall avoid those air-circulation locations such as air conditioning, windows and doors.
5.2.2 PLACE the nutrient agar plate at the sampling point, OPEN the dish lid, EXPOSE
for 5min, COVER the dish lid, FLIP the plate, PLACE into 36ºC ± 1ºC thermostat incubator
to INCUBATE for 48 h.
5.2.3 COUNT the number of colonies grown on each plate, CALCULATE the average
number of colonies of all sampling points. The number of colonies per petri dish (cfu/DISH)
shall be reported as the result.
GB/T 18204.1-2000
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
Methods of microbiological examination for air in
public places -
Determination of aerobic bacterial count
ISSUED ON. SEPTEMBER 30, 2000
IMPLEMENTED ON. JANUARY 01, 2001
Issued by. National Quality and Technical Supervision Bureau
GB
Tips - GB 18204 Series (not part of this Standard)
Standard Description Issued Date
Enforced
Date Newer Version (Click to Check)
GB/T 18204.1-2000 Methods of microbiological examination for air in public places - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.2-2000 Methods of microbiological examination for tea set in public places - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.3-2000 Methods of microbiological examination for tea set in public places - Determination of coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.4-2000 Methods of microbiological examination for towel and bedclothes in public places - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.5-2000 Methods of microbiological examination for towel and bedclothes in public places - Determination of Coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.6-2000 Methods of microbiological examination for barber's tools - Determination of Coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.7-2000 Methods of microbiological examination for barber's tools - Determination of Staphylococcus aureus 2000-09-30 2001-01-01
GB/T 18204.8-2000 Methods of microbiological examination for slippers in public places - Determination of molds and yeasts count 2000-09-30 2001-01-01
GB/T 18204.9-2000 Methods of microbiological examination for water in swimming pool - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.10-2000 Methods of microbiological examination for water in swimming pool - Determination of Coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.11-2000 Methods of microbiological examination for bath tube and wash basin in public places - Determination of aerobic bacterial count 2000-09-30 2001-01-01
GB/T 18204.12-2000 Methods of microbiological examination for bath tube and wash basin in public places - Determination of Coliform bacteria 2000-09-30 2001-01-01
GB/T 18204.16-2000 Method for determination of atmospheric pressure in public places 2000-09-30 2001-01-01
GB/T 18204.18-2000 Method for determination of air change flow of indoor air in public places 2000-09-30 2001-01-01
GB/T 18204.19-2000 Method for measurement of infiltration rates of indoor air in public places 2000-09-30 2001-01-01
GB/T 18204.23-2000 Methods for determination of carbon monoxide in air of public places 2000-09-30 2001-01-01
GB/T 18204.28-2000 Method for determination of temperature in water of swimming place 2000-09-30 2001-01-01
GB/T 18204.1-2013 Hygiene inspection methods in public places - Part 1. Physical factors 2014-12-01
GB/T 18204.3-2013 Hygiene inspection methods in public places - Part 3. Airborne microbes 2014-12-01
GB/T 18204.4-2013 Hygiene inspection methods in public places - Part 4. Public appliances microorganisms 2014-12-01
GB/T 18204.5-2013 Hygiene inspection methods in public places - Part 5. Air conditioning system 2014-12-01
GB/T 18204.6-2013 Hygiene inspection methods in public places - Part 6. Health monitoring technical specifications 2014-12-01
Table of Contents
Foreword ... 6
1 Scope ... 7
2 Definitions... 7
3 Instruments and devices ... 7
4 Medium ... 8
5 Operational steps ... 8
Foreword
In order to implement the "Public Places Hygienic Management Regulations" and GB
9663~9673-1996, GB 16153-1996 "Public Places Hygienic Standard”, and to strengthen
the hygienic supervision and management of public places, this Standard is formulated.
The methods in this Standard are the supervision and inspection methods supporting to
GB 9663~9673-1996.
The first method of this Standard is the arbitration method.
This Standard is released for the first-time.
This Standard is proposed by Ministry of Health, the People's Republic of China.
Drafting organizations of this Standard. Chinese Academy of Preventive Medicine and
Environmental Hygiene Monitoring Institute, Beijing Municipal Health and Epidemic
Prevention Station, Guangdong Provincial Health and Epidemic Prevention Station, and
Tianjing Municipal Health and Epidemic Prevention Station.
The main drafters of this Standard. Zhou Shuyu, Chen Xiping, Gao Hui, Cai Han, and
Zhang Shulan.
Methods of microbiological examination for air in public places -
Determination of aerobic bacterial count
1 Scope
This Standard specifies the determination method of total number of bacteria in the air of
public places.
This Standard applies to the determination of total number of bacteria in the air of public
places.
2 Definitions
This Standard uses the following definitions.
2.1 Impacting method
It refers to the sampling determination method - USE percussive air microbial sampler to
sample; through pumping dynamic action, LET air go through slits or small holes so as to
generate high-speed airflow; so that the bacteria-carrying particles suspended in air hit
onto nutrient agar plate; after incubating at 37ºC for 48 h; CALCULATE the number of
bacterial colonies contained in each cubic meter of air.
2.2 Natural sinking method
It refers to the sampling determination method - A nutrient agar plate, diameter of 9 cm, is
exposed for 5 min at the sampling point; after incubating at 37ºC for 48 h; CALCULATE
the number of grown bacterial colonies.
3 Instruments and devices
3.1 High-pressure steam sterilizer.
3.2 Dry-heat Sterilizer.
3.3 Thermostat incubator.
3.4 Refrigerator.
3.5 Petri dish (diameter of 9 cm).
3.6 General devices for preparing medium. Measuring bottle, conical flask, pH meter or
precision pH paper, etc.
3.7 Percussive air microbial sampler.
and shall avoid those air-circulation locations such as air conditioning, windows and doors.
5.2.2 PLACE the nutrient agar plate at the sampling point, OPEN the dish lid, EXPOSE
for 5min, COVER the dish lid, FLIP the plate, PLACE into 36ºC ± 1ºC thermostat incubator
to INCUBATE for 48 h.
5.2.3 COUNT the number of colonies grown on each plate, CALCULATE the average
number of colonies of all sampling points. The number of colonies per petri dish (cfu/DISH)
shall be reported as the result.