GB/T 14455.3-2008 English PDF (GBT14455.3-2008)

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GB/T 14455.3-2008: Fragrance/Flavor substances -- Evaluation of solubility (miscibility) in ethanol
GB/T 14455.3-2008
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 71.100.60
Y 41
Replacing GB/T 14455.3-1993, GB/T 14457.1-1993
Fragrance / Flavor Substances -
Evaluation of Solubility (Miscibility) in Ethanol
(ISO 875:1999, Essential Oils - Evaluation of Miscibility in Ethanol, MOD)
ISSUED ON: JULY 15, 2008
IMPLEMENTED ON: NOVEMBER 1, 2008
Issued by: General Administration of Quality Supervision, Inspection and
Quarantine;
Standardization Administration of the People’s Republic of
China.
Table of Contents
Foreword ... 3 
1 Scope ... 5 
2 Normative References ... 5 
3 Principle ... 5 
4 Classification of Miscibility ... 6 
5 Reagents ... 6 
6 Instruments ... 7 
7 Sample Preparation ... 8 
8 Operating Procedures ... 8 
9 Expression of Results ... 9 
10 Test Report ... 10 
Fragrance / Flavor Substances -
Evaluation of Solubility (miscibility) in Ethanol
1 Scope
This Part of GB/T 14455 specifies the method for the evaluation of miscibility of
essential oils in aqueous ethanol solution with an already-known content and the
method for the determination of solubility of isolate and synthetic fragrances in
aqueous ethanol solution with an already-known content.
This Part is applicable to the evaluation of miscibility of essential oils and the
determination of solubility of isolate and synthetic fragrances.
2 Normative References
Through the reference in this Part of GB/T 14455, the clauses of the following
documents become clauses of this Part. In terms of references with a specified date,
all the subsequent modification sheets (excluding the corrected content) or revised
versions are not applicable to this Part. However, the various parties that reach an
agreement in accordance with this Part are encouraged to explore the possibility of
adopting the latest version of these documents. In terms of references without a
specified date, the latest version is applicable to this Part.
GB/T 14454.1 Fragrance / Flavor Substances - Preparation of Test Samples (GB/T
14454.1-2008, ISO 356:1996, MOD)
3 Principle
3.1 Miscibility of Essential Oils in Ethanol
At 20 °C, gradually add aqueous ethanol solution of an appropriate concentration to
essential oils; evaluate the miscibility and possible lacteous phenomenon.
3.2 Solubility of Isolate and Synthetic Fragrances in Ethanol
At 25 °C, various isolate and synthetic fragrances in different concentrations of
aqueous ethanol solution have different solubilities.
NOTE: solubility refers to the milliliters of aqueous ethanol solution required when at a
specified temperature, 1 mL or 1 g of isolate and synthetic fragrances are
completely solubilized in a certain concentration of aqueous ethanol solution.
4 Classification of Miscibility
4.1 At 20 °C, when a volume of certain essential oil is mixed with V volume of aqueous
ethanol solution with a certain concentration, and then, becomes clear and transparent,
then, gradually add the aqueous ethanol solution of that concentration, till the volume
of ethanol becomes 20, if it can still remain clear and transparent, then, it is believed
that this essential oil is miscible with V volume or a larger volume of aqueous ethanol
solution with that concentration.
4.2 At 20 °C, when a volume of certain essential oil is mixed with V volume of aqueous
ethanol solution with a certain concentration, and then, becomes clear and transparent,
but after gradually adding (V-V) volume of aqueous ethanol solution with that
concentration, it becomes turbid; and when the volume of ethanol becomes 20, it still
remains turbid, then, it is believed that this essential oil is miscible with V volume of
aqueous ethanol solution with that concentration, and becomes turbid when it is diluted
to the volume of V.
4.3 At 20 °C, when a volume of certain essential oil is mixed with V volume of aqueous
ethanol solution with a certain concentration, and then, becomes clear and transparent,
but after gradually adding (V-V) volume of aqueous ethanol solution with that
concentration, it becomes turbid; and after further adding (V-V) volume of aqueous
ethanol solution with that concentration, it becomes clear and transparent, then, it is
believed that this essential oil is miscible with V volume of aqueous ethanol solution
with that concentration, and becomes turbid when it is diluted to the volume of V, till it
reaches V.
4.4 After mixing an essential oil with a certain concentration of aqueous ethanol
solution (accordance with the conditions specified in 4.1, 4.2 and 4.3), it becomes
lacteous. If this lacteous color is the same as the freshly prepared lacteous standard
solution specified in 5.3, then, it is believed that this essential oil is miscible with this
concentration of aqueous ethanol solution to form a lacteous solution.
NOTE: the value of V, Vand V are not more than 20.
5 Reagents
All the used reagents are analytically pure; water is distilled water or water of equivalent
purity.
5.1 95% (volume fraction) ethanol.
5.2 Aqueous ethanol solution: generally used aqueous ethanol solution, the content of
ethanol (volume fraction) is: 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% and
95%.
5.3.
8.3 Determination of Solubility of Isolate and Synthetic Fragrances
Use burette (6.1) to add aqueous ethanol solution (5.2) which is pre-adjusted to 25 °C
± 0.2 °C with an already-known concentration to the sample. Each time, add 0.1 mL;
vigorously shake it, till it completely solubilizes. When the solution becomes clear and
transparent, record the milliliters of the added aqueous ethanol solution, which is the
solubility. Or, in accordance with the stipulations of solubility in product standards
regarding fragrances, at a time, add aqueous ethanol solution of specified
concentration and volume; preserve the heat and shake it for a while. If a clear and
transparent solution is obtained, then, it shall be qualified.
NOTE 1: the determination of solubility generally uses ethanol as the solvent. When other
solvents are used, it shall be pointed out in the product standards regarding
fragrances.
NOTE 2: during the determination, if a certain concentration of aqueous ethanol solution
is added to 10 mL, the clear and transparent solution still cannot be obtained,
then, use a more concentrated level of aqueous ethanol solution in Table 1 for
re-determination.
9 Expression of Results
9.1 Miscibility of Essential Oils
At 20 °C, the miscibility of essential oils and aqueous ethanol solution (see Table 1)
with the concentration of Q is expressed as follows:
a) Circumstance 1 (4.1):
1 volume of essential oil solubilizes in V volume of aqueous ethanol solution
with the concentration of Q;
b) Circumstance 2 (4.2):
1 volume of essential oil solubilizes in V volume of aqueous ethanol solution
with the concentration of Q, while manifests turbidity in V volume of aqueous
ethanol solution with the same concentration;
c) Circumstance 3 (4.3):
1 volume of essential oil solubilizes in V volume of aqueous ethanol solution
with the concentration of Q, while manifests turbidity in V to V volume of
aqueous ethanol solution with the same concentration;
In which,