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See Chinese contents: GB 4789.49-2024 GB 4789.49-2024: National Food Safety Standards--Food Microbiological Testing--Testing for Shiga toxin-producing Escherichia coli
This standard specifies the testing methods for Shiga toxin-producing Escherichia coli (STEC) in food. This standard is applicable to the testing for Shiga toxin-producing Escherichia coli in food. 2 Equipment and materials In addition to routine sterilization and culture equipment for microbiology laboratories, other equipment and materials are as follows. 2.1 Constant temperature incubator: 36 ¡æ¡À1 ¡æ, 44 ¡æ¡À1 ¡æ. 2.2 Refrigerator: 2 ¡æ~5 ¡æ, -18 ¡æ~-20 ¡æ. 2.3 Balance: The sensitivity is 0.001 g. 2.4 Homogenizer. 2.5 Vortex mixer. 2.6 Constant temperature water bath of 50 ¡æ~55 ¡æ, and constant temperature metal bath of 99 ¡æ¡À1 ¡æ. 2.7 Centrifuge: The maximum speed is at least 16000 g. 2.8 Real-time fluorescence quantitative PCR instrument. 2.9 Mixer. 2.10 Sterile pipette: 1 mL (with a scale of 0.01 mL), 10 mL (with a scale of 0.1 mL). 2.11 Electronic pipette or ear bulb. 2.12 Sterile conical flask with stopper: The capacities are 250 mL, 500 mL, and 1000 mL. 2.13 Sterile culture dish: 90 mm in diameter. GB 4789.49-2024 www.ChineseStandard.net ? Buy True-PDF ? Auto-delivery Page 4 of 17 2.14 Sterile homogenizing bag: with a filter and a sterile homogenizing cup. 2.15 Flat-cap 8-tube strips or 96-well PCR microplates. 2.16 Magnetic bead separation and enrichment device. 2.17 Micropipette and tip: 0.2 ¦ÌL~2 ¦ÌL, 2 ¦ÌL~20 ¦ÌL, 20 ¦ÌL~200 ¦ÌL, and 100 ¦ÌL~1000 ¦ÌL. 2.18 10
