GB 4789.3-2010 English PDF

GB 4789.3-2010: National food safety standard -- Food microbiological examination: Enumeration of coliforms
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GB 4789.3-2010
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard
Food Microbiological Examination.
Enumeration of Coliforms
ISSUED ON. MARCH 26, 2010
IMPLEMENTED ON. JUNE, 1, 2010
Issued by. Ministry of Health of the People’s Republic of China
GB
Tips - GB 4789 Series (Not part of this Standard)
Standard ID Standard Name Issued Date
Enforced
Date New Version (Click to check)?
GB 4789.1-2010 National food safety standard Food microbiological examination. General guidelines 2010-03-26 2010-06-01
GB 4789.2-2010 National food safety standard Food microbiological examination. Aerobic plate count 2010-03-26 2010-06-01
GB 4789.3-2010 National food safety standard Food microbiological examination. Enumeration of coliforms 2010-03-26 2010-06-01
GB 4789.4-2010 National food safety standard Food microbiological examination. Salmonella 2010-03-26 2010-06-01
GB 4789.5-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Shigella 2012-05-17 2012-07-17
GB/T 4789.6-
Microbiological examination of food hygiene Examination of
diarrheogenic Escherichia coli 2003-08-11 2004-01-01
GB/T 4789.7-
Microbiological examination of food hygiene. Examination of
Vibrio parahaemolyticus 2008-05-16 2008-11-01
GB/T 4789.8-
Microbiological examination of food hygiene. Examination of
Yersinia enterocolitica 2008-11-21 2009-03-01
GB/T 4789.9-
Microbiological examination of food hygiene. Examination of
Campylobacter jejuni 2008-11-21 2009-03-01
GB 4789.10-2010 National food safety standard Food microbiological examination. Staphylococcus aureus 2008-11-21 2009-03-01
GB/T 4789.11-
Microbiological examination of food hygiene Examination of
streptococcus hemolyticus 2003-08-11 2004-01-01
GB/T 4789.12-
Microbiological examination of food hygiene Examination of
Clostridium botulinum and botulinus toxin 2003-08-11 2004-01-01
GB 4789.13-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Clostridium Perfringens 2012-05-17 2012-07-17
GB/T 4789.14-
Microbiological examination of food hygiene Examination of
Bacillus cereus 2003-08-11 2004-01-01
GB 4789.15-2010 National food safety standard Food microbiological examination. Enumeration of moulds and yeasts 2010-03-26 2010-06-01
GB/T 4789.16-
Microbiological examination of food hygiene Identification of
common mycotoxin producing fungi 2003-08-11 2004-01-01
GB/T 4789.17-
Microbiological examination of food hygiene Examination of
meat and meat products 2003-08-11 2004-01-01
GB 4789.18-2010 National food safety standard Food microbiological examination. Milk and milk products 2010-03-26 2010-06-01
GB/T 4789.19-
Microbiological examination of food hygiene Examination of
egg and egg products 2003-08-11 2004-01-01
GB/T 4789.20-
Microbiological examination of food hygiene Examination of
aquatic product foods 2003-08-11 2004-01-01
GB/T 4789.21-
Microbiological examination of food hygiene Examination of
frozen drinks and cold drinks 2003-08-11 2004-01-01
GB/T 4789.22-
Microbiological examination of food hygiene Examination of
flavourings 2003-08-11 2004-01-01
GB/T 4789.23-
Microbiological examination of food hygiene Examination of
cold dish and bean products 2003-08-11 2004-01-01
GB/T 4789.24-
Microbiological examination of food hygiene Examination of
candy, cake and preserved fruits 2003-08-11 2004-01-01
GB/T 4789.25-
Microbiological examination of food hygiene Examination of
wines 2003-08-11 2004-01-01
GB/T 4789.26-
Microbiological examination of food hygiene Examination of
commercial sterilization of canned food 2003-08-11 2004-01-01
GB/T 4789.27-
Microbiological examination of food hygiene. Examination of
residue of antibiotics in fresh milk 2008-11-21 2009-03-01
GB/T 4789.28-
Microbiological examination of food hygiene Staining
methods, culture mediums and reagents 2003-08-11 2004-01-01
GB/T 4789.29-
Microbiological examination of food hygiene Examination of
pseudomonas cocovenenans subsp. farinofermentans 2003-08-11 2004-01-01
GB 4789.30-2010 National food safety standard Food microbiological examination. Listeria monocytogenes 2010-03-26 2010-06-01
GB/T 4789.31-
Microbiological examination of food hygiene Examination of
salmonellae, shigellae, and diarrhoea causative Escherichia
coli by means of the diagnostic typing phage set for
enterobacteriaceae
GB/T 4789.32-
Microbiological examination for food hygiene rapid detection
of coliform bacteria 2002-06-13 2002-10-01
GB/T 4789.33-
Microbiological examination of food hygiene Examination of
cereal, fruit and vegetable 2003-08-11 2004-01-01
GB 4789.34-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Bifidobacterium 2012-05-17 2012-07-17
GB 4789.35-2010 National food safety standard Food microbiological examination. Lactic acid bacteria 2010-03-26 2010-06-01
GB/T 4789.36-
Microbiological examination of food hygiene. Examination of
Escherichia coli O157. H7/NM 2008-05-16 2008-11-01
GB/T 4789.37-
Microbiological examination of food hygiene. Examination of
Staphylococcus aureus 2008-11-21 2009-03-01
GB 4789.38-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Enumeration of Escherichia Coli 2012-05-17 2012-07-17
GB/T 4789.39-
Microbiological examination of food hygiene. Enumeration of
faecal coliforms 2008-11-21 2009-03-01
GB 4789.40-2010 National food safety standard Food microbiological examination. Enterobacter sakazakii 2010-03-26 2010-06-01
Table of Contents
Foreword ... 7
1 Scope ... 8
2 Terms and Definitions ... 8
3 Devices and Materials... 8
4 Culture Medium and Reagents ... 9
5 Examination Procedure ... 9
6 Operational Procedure ... 10
7 Examination Procedure ... 12
8 Operational Procedure ... 12
Annex A ... 14
Annex B ... 17
Foreword
This Standard replaces GB/T 4789.3-2008 Microbiological Examination of Food Hygiene –
Enumeration of Coliforms.
The main changes in this Standard over GB/T 4789.3-2008 are as follows.
- Modify standard name of both English and Chinese;
- Modify the selection range of plate colony count of “Method 2. Coliform Plate Count
Method” into “15 CFU ~ 150 CFU”;
- Delete “Method 3. Coliforms PertifilmTM Test Strip Method”.
Annex A and Annex B of this Standard are normative.
The historical editions replaced by this Standard are as follows.
- GB 4789.3-1984, GB 4789.3-1994, GB 4789.3-2003, and GB 4789.3-2008.
National food safety standard Food microbiological
examination. Enumeration of Coliforms
1 Scope
This Standard specifies the methods of enumeration of coliforms in food.
This Standard is applicable to the enumeration of coliforms in food.
2 Terms and Definitions
2 . 1 Coliforms
Aerobic and facultative anaerobic gram-negative asporous bacilli that can ferment
lactose and are acid-producing and aerogenic, under certain culture conditions.
2 . 2 Most probable number (MPN)
An indirect enumeration method based on Poisson distribution.
3 Devices and Materials
In addition to conventional sterilization and culture devices in the microbiology
laboratory, other devices and materials are as follows.
3 . 1 Thermostat incubator. 36 ºC ± 1 ºC.
3 . 2 Refrigerator. 2 ºC ~ 5 ºC.
3 . 3 Thermostat water bath. 46 ºC ± 1 ºC.
3 . 4 Balance. sensibility 0.1 g.
3 . 5 Homogenizer.
3 . 6 Oscillator.
3 . 7 Sterile sucking tubes. 1 mL (accurate to 0.01 mL), 10 mL (accurate to 0.1 mL) or
micropipette with a sucker.
3 . 8 Sterile conical flask. 500 mL.
3 . 9 Sterile petri dish. diameter 90 mm.
3 . 1 0 pH meter or pH cuvette tube or precision pH test paper.
3 . 1 1 Colony counter.
8 . 3 S e l e c t i o n o f p l a t e c o l o n y c o u n t
Select the plates with colony counts of 15 CFU – 150 CFU and enumerate typical
and suspicious coliform colonies on the plates respectively. Typical colonies are
claret, around which is zone of precipitated red bile salt. The colonies have a
diameter of 0.5 mm or above.
8 . 4 C o n f i r m a t i o n t e s t
Pick up 10 typical and suspicious colonies of different types from the plates, then
inoculate into BGLB broth tubes respectively, culture at 36 ºC ± 1 ºC for 24 h – 48 h
and observe the aerogenesis. The BGLB broth tubes with aerogenesis can be
...
GB 4789.3-2010
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard
Food Microbiological Examination.
Enumeration of Coliforms
ISSUED ON. MARCH 26, 2010
IMPLEMENTED ON. JUNE, 1, 2010
Issued by. Ministry of Health of the People’s Republic of China
GB
Tips - GB 4789 Series (Not part of this Standard)
Standard ID Standard Name Issued Date
Enforced
Date New Version (Click to check)?
GB 4789.1-2010 National food safety standard Food microbiological examination. General guidelines 2010-03-26 2010-06-01
GB 4789.2-2010 National food safety standard Food microbiological examination. Aerobic plate count 2010-03-26 2010-06-01
GB 4789.3-2010 National food safety standard Food microbiological examination. Enumeration of coliforms 2010-03-26 2010-06-01
GB 4789.4-2010 National food safety standard Food microbiological examination. Salmonella 2010-03-26 2010-06-01
GB 4789.5-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Shigella 2012-05-17 2012-07-17
GB/T 4789.6-
Microbiological examination of food hygiene Examination of
diarrheogenic Escherichia coli 2003-08-11 2004-01-01
GB/T 4789.7-
Microbiological examination of food hygiene. Examination of
Vibrio parahaemolyticus 2008-05-16 2008-11-01
GB/T 4789.8-
Microbiological examination of food hygiene. Examination of
Yersinia enterocolitica 2008-11-21 2009-03-01
GB/T 4789.9-
Microbiological examination of food hygiene. Examination of
Campylobacter jejuni 2008-11-21 2009-03-01
GB 4789.10-2010 National food safety standard Food microbiological examination. Staphylococcus aureus 2008-11-21 2009-03-01
GB/T 4789.11-
Microbiological examination of food hygiene Examination of
streptococcus hemolyticus 2003-08-11 2004-01-01
GB/T 4789.12-
Microbiological examination of food hygiene Examination of
Clostridium botulinum and botulinus toxin 2003-08-11 2004-01-01
GB 4789.13-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Clostridium Perfringens 2012-05-17 2012-07-17
GB/T 4789.14-
Microbiological examination of food hygiene Examination of
Bacillus cereus 2003-08-11 2004-01-01
GB 4789.15-2010 National food safety standard Food microbiological examination. Enumeration of moulds and yeasts 2010-03-26 2010-06-01
GB/T 4789.16-
Microbiological examination of food hygiene Identification of
common mycotoxin producing fungi 2003-08-11 2004-01-01
GB/T 4789.17-
Microbiological examination of food hygiene Examination of
meat and meat products 2003-08-11 2004-01-01
GB 4789.18-2010 National food safety standard Food microbiological examination. Milk and milk products 2010-03-26 2010-06-01
GB/T 4789.19-
Microbiological examination of food hygiene Examination of
egg and egg products 2003-08-11 2004-01-01
GB/T 4789.20-
Microbiological examination of food hygiene Examination of
aquatic product foods 2003-08-11 2004-01-01
GB/T 4789.21-
Microbiological examination of food hygiene Examination of
frozen drinks and cold drinks 2003-08-11 2004-01-01
GB/T 4789.22-
Microbiological examination of food hygiene Examination of
flavourings 2003-08-11 2004-01-01
GB/T 4789.23-
Microbiological examination of food hygiene Examination of
cold dish and bean products 2003-08-11 2004-01-01
GB/T 4789.24-
Microbiological examination of food hygiene Examination of
candy, cake and preserved fruits 2003-08-11 2004-01-01
GB/T 4789.25-
Microbiological examination of food hygiene Examination of
wines 2003-08-11 2004-01-01
GB/T 4789.26-
Microbiological examination of food hygiene Examination of
commercial sterilization of canned food 2003-08-11 2004-01-01
GB/T 4789.27-
Microbiological examination of food hygiene. Examination of
residue of antibiotics in fresh milk 2008-11-21 2009-03-01
GB/T 4789.28-
Microbiological examination of food hygiene Staining
methods, culture mediums and reagents 2003-08-11 2004-01-01
GB/T 4789.29-
Microbiological examination of food hygiene Examination of
pseudomonas cocovenenans subsp. farinofermentans 2003-08-11 2004-01-01
GB 4789.30-2010 National food safety standard Food microbiological examination. Listeria monocytogenes 2010-03-26 2010-06-01
GB/T 4789.31-
Microbiological examination of food hygiene Examination of
salmonellae, shigellae, and diarrhoea causative Escherichia
coli by means of the diagnostic typing phage set for
enterobacteriaceae
GB/T 4789.32-
Microbiological examination for food hygiene rapid detection
of coliform bacteria 2002-06-13 2002-10-01
GB/T 4789.33-
Microbiological examination of food hygiene Examination of
cereal, fruit and vegetable 2003-08-11 2004-01-01
GB 4789.34-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Bifidobacterium 2012-05-17 2012-07-17
GB 4789.35-2010 National food safety standard Food microbiological examination. Lactic acid bacteria 2010-03-26 2010-06-01
GB/T 4789.36-
Microbiological examination of food hygiene. Examination of
Escherichia coli O157. H7/NM 2008-05-16 2008-11-01
GB/T 4789.37-
Microbiological examination of food hygiene. Examination of
Staphylococcus aureus 2008-11-21 2009-03-01
GB 4789.38-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Enumeration of Escherichia Coli 2012-05-17 2012-07-17
GB/T 4789.39-
Microbiological examination of food hygiene. Enumeration of
faecal coliforms 2008-11-21 2009-03-01
GB 4789.40-2010 National food safety standard Food microbiological examination. Enterobacter sa...