GB 4789.2-2010 English PDF

GB 4789.2-2010: National food safety standard -- Food microbiological examination: Aerobic plate count
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GB 4789.2-2010
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard
Food Microbiological Examination.
Aerobic Plate Count
ISSUED ON. MARCH 26, 2010
IMPLEMENTED ON. JUNE, 1, 2010
Issued by. Ministry of Health of the People’s Republic of China
GB
Tips - GB 4789 Series (Not part of this Standard)
Standard ID Standard Name Issued Date
Enforced
Date New Version (Click to check)?
GB 4789.1-2010 National food safety standard Food microbiological examination. General guidelines 2010-03-26 2010-06-01
GB 4789.2-2010 National food safety standard Food microbiological examination. Aerobic plate count 2010-03-26 2010-06-01
GB 4789.3-2010 National food safety standard Food microbiological examination. Enumeration of coliforms 2010-03-26 2010-06-01
GB 4789.4-2010 National food safety standard Food microbiological examination. Salmonella 2010-03-26 2010-06-01
GB 4789.5-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Shigella 2012-05-17 2012-07-17
GB/T 4789.6-
Microbiological examination of food hygiene Examination of
diarrheogenic Escherichia coli 2003-08-11 2004-01-01
GB/T 4789.7-
Microbiological examination of food hygiene. Examination of
Vibrio parahaemolyticus 2008-05-16 2008-11-01
GB/T 4789.8-
Microbiological examination of food hygiene. Examination of
Yersinia enterocolitica 2008-11-21 2009-03-01
GB/T 4789.9-
Microbiological examination of food hygiene. Examination of
Campylobacter jejuni 2008-11-21 2009-03-01
GB 4789.10-2010 National food safety standard Food microbiological examination. Staphylococcus aureus 2008-11-21 2009-03-01
GB/T 4789.11-
Microbiological examination of food hygiene Examination of
streptococcus hemolyticus 2003-08-11 2004-01-01
GB/T 4789.12-
Microbiological examination of food hygiene Examination of
Clostridium botulinum and botulinus toxin 2003-08-11 2004-01-01
GB 4789.13-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Clostridium Perfringens 2012-05-17 2012-07-17
GB/T 4789.14-
Microbiological examination of food hygiene Examination of
Bacillus cereus 2003-08-11 2004-01-01
GB 4789.15-2010 National food safety standard Food microbiological examination. Enumeration of moulds and yeasts 2010-03-26 2010-06-01
GB/T 4789.16-
Microbiological examination of food hygiene Identification of
common mycotoxin producing fungi 2003-08-11 2004-01-01
GB/T 4789.17-
Microbiological examination of food hygiene Examination of
meat and meat products 2003-08-11 2004-01-01
GB 4789.18-2010 National food safety standard Food microbiological examination. Milk and milk products 2010-03-26 2010-06-01
GB/T 4789.19-
Microbiological examination of food hygiene Examination of
egg and egg products 2003-08-11 2004-01-01
GB/T 4789.20-
Microbiological examination of food hygiene Examination of
aquatic product foods 2003-08-11 2004-01-01
GB/T 4789.21-
Microbiological examination of food hygiene Examination of
frozen drinks and cold drinks 2003-08-11 2004-01-01
GB/T 4789.22-
Microbiological examination of food hygiene Examination of
flavourings 2003-08-11 2004-01-01
GB/T 4789.23-
Microbiological examination of food hygiene Examination of
cold dish and bean products 2003-08-11 2004-01-01
GB/T 4789.24-
Microbiological examination of food hygiene Examination of
candy, cake and preserved fruits 2003-08-11 2004-01-01
GB/T 4789.25-
Microbiological examination of food hygiene Examination of
wines 2003-08-11 2004-01-01
GB/T 4789.26-
Microbiological examination of food hygiene Examination of
commercial sterilization of canned food 2003-08-11 2004-01-01
GB/T 4789.27-
Microbiological examination of food hygiene. Examination of
residue of antibiotics in fresh milk 2008-11-21 2009-03-01
GB/T 4789.28-
Microbiological examination of food hygiene Staining
methods, culture mediums and reagents 2003-08-11 2004-01-01
GB/T 4789.29-
Microbiological examination of food hygiene Examination of
pseudomonas cocovenenans subsp. farinofermentans 2003-08-11 2004-01-01
GB 4789.30-2010 National food safety standard Food microbiological examination. Listeria monocytogenes 2010-03-26 2010-06-01
GB/T 4789.31-
Microbiological examination of food hygiene Examination of
salmonellae, shigellae, and diarrhoea causative Escherichia
coli by means of the diagnostic typing phage set for
enterobacteriaceae
GB/T 4789.32-
Microbiological examination for food hygiene rapid detection
of coliform bacteria 2002-06-13 2002-10-01
GB/T 4789.33-
Microbiological examination of food hygiene Examination of
cereal, fruit and vegetable 2003-08-11 2004-01-01
GB 4789.34-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Bifidobacterium 2012-05-17 2012-07-17
GB 4789.35-2010 National food safety standard Food microbiological examination. Lactic acid bacteria 2010-03-26 2010-06-01
GB/T 4789.36-
Microbiological examination of food hygiene. Examination of
Escherichia coli O157. H7/NM 2008-05-16 2008-11-01
GB/T 4789.37-
Microbiological examination of food hygiene. Examination of
Staphylococcus aureus 2008-11-21 2009-03-01
GB 4789.38-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Enumeration of Escherichia Coli 2012-05-17 2012-07-17
GB/T 4789.39-
Microbiological examination of food hygiene. Enumeration of
faecal coliforms 2008-11-21 2009-03-01
GB 4789.40-2010 National food safety standard Food microbiological examination. Enterobacter sakazakii 2010-03-26 2010-06-01
Table of Contents
Foreword ...7
1 Scope ...8
2 Terms and definitions ...8
3 Devices and materials ...8
4 Culture medium and reagents ...8
5 Examination procedure ...9
6 Operational procedure ...9
7 Result and reporting ... 11
Annex A ...14
Foreword
This Standard replaces GB/T 4789.2-2008 Microbiological Examination of Food Hygiene -
Aerobic Plate Count.
The main changes in this Standard over GB/T 4789.2-2008 are as follows.
- Modify the standard name of both English and Chinese;
- Modify the explanation of the calculation formula for aerobic plate count;
- Modify the culture medium and reagents;
- Delete the “second method - aerobic plate count PetrifilmTM test strip method”.
Annex A of this Standard is normative.
The historical editions replaced by this Standard are as follows.
- GB 4789.2-1984, GB 4789.2-1994, GB/T 4789.2-2003, and GB/T 4789.2-2008.
National food safety standard Food microbiological
examination. Aerobic plate count
1 Scope
This Standard specifies determination methods of aerobic plate count.
This Standard is applicable to the determination of aerobic plate count.
2 Terms and definitions
2.1 Aerobic plate count
It refers to the total number of microbes colonies formed in per g (mL) of testing
sample obtained from the food which is processed, and cultured (such as culture
medium, culture temperature and incubation time) under certain conditions.
3 Devices and materials
Except the conventional sterilization and culture devices of microbiological
laboratory, other devices and materials are as follows.
3.1 Thermostat incubator. 36 ºC ± 1 ºC, 30 ºC ± 1 ºC.
3.2 Refrigerator. 2 ºC.
3.3 Thermostat water bath. 46 ºC± 1 ºC.
3.4 Balance. sensitivity 0.1g.
3.5 Homogenizer.
3.6 Oscillator.
3.7 Sterile pipettes. 1 mL (accurate to 0.01 mL), 10 mL (accurate to 0.1 mL) or micro-
pipettes with sucking head.
3.8 Sterile conical flasks. 250 mL and 500 mL.
3.9 Sterile petri dish. 90 mm in diameter.
3.10 pH meter or pH cuvette tube or precision pH test paper.
3.11 Magnifier or/and colony counter.
4 Culture medium and reagents
medium (about 4 mL), after the solidification, reverse the petri dish and make
culture under the equal conditions specified in 6.2.1.
6 . 3 Colony count
Perform visual observation, use magnifier or colony counter (if necessary), and
record the dilution fold and the count of corresponding colonies. The colony count
is represented as colony-forming units (CFU).
6.3.1 Select the aerobic plates with the colony count ranging from 30 CFU to 300 CFU
and without diffused growth of colonies to calculate the total colony count. For the
plates lower than 30 CFU, record the exact colony number, and the plates with
more than 300 CFU can be recorded as “countless”. The colony count of each
dilution shall be the average of two plate counts.
6 . 3 . 2 If one of the plates has colonies growing in a comparatively large patch, it should
not be used and the other plate free of this problem shall be used for the colony
count of the corr...
GB 4789.2-2010
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard
Food Microbiological Examination.
Aerobic Plate Count
ISSUED ON. MARCH 26, 2010
IMPLEMENTED ON. JUNE, 1, 2010
Issued by. Ministry of Health of the People’s Republic of China
GB
Tips - GB 4789 Series (Not part of this Standard)
Standard ID Standard Name Issued Date
Enforced
Date New Version (Click to check)?
GB 4789.1-2010 National food safety standard Food microbiological examination. General guidelines 2010-03-26 2010-06-01
GB 4789.2-2010 National food safety standard Food microbiological examination. Aerobic plate count 2010-03-26 2010-06-01
GB 4789.3-2010 National food safety standard Food microbiological examination. Enumeration of coliforms 2010-03-26 2010-06-01
GB 4789.4-2010 National food safety standard Food microbiological examination. Salmonella 2010-03-26 2010-06-01
GB 4789.5-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Shigella 2012-05-17 2012-07-17
GB/T 4789.6-
Microbiological examination of food hygiene Examination of
diarrheogenic Escherichia coli 2003-08-11 2004-01-01
GB/T 4789.7-
Microbiological examination of food hygiene. Examination of
Vibrio parahaemolyticus 2008-05-16 2008-11-01
GB/T 4789.8-
Microbiological examination of food hygiene. Examination of
Yersinia enterocolitica 2008-11-21 2009-03-01
GB/T 4789.9-
Microbiological examination of food hygiene. Examination of
Campylobacter jejuni 2008-11-21 2009-03-01
GB 4789.10-2010 National food safety standard Food microbiological examination. Staphylococcus aureus 2008-11-21 2009-03-01
GB/T 4789.11-
Microbiological examination of food hygiene Examination of
streptococcus hemolyticus 2003-08-11 2004-01-01
GB/T 4789.12-
Microbiological examination of food hygiene Examination of
Clostridium botulinum and botulinus toxin 2003-08-11 2004-01-01
GB 4789.13-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Clostridium Perfringens 2012-05-17 2012-07-17
GB/T 4789.14-
Microbiological examination of food hygiene Examination of
Bacillus cereus 2003-08-11 2004-01-01
GB 4789.15-2010 National food safety standard Food microbiological examination. Enumeration of moulds and yeasts 2010-03-26 2010-06-01
GB/T 4789.16-
Microbiological examination of food hygiene Identification of
common mycotoxin producing fungi 2003-08-11 2004-01-01
GB/T 4789.17-
Microbiological examination of food hygiene Examination of
meat and meat products 2003-08-11 2004-01-01
GB 4789.18-2010 National food safety standard Food microbiological examination. Milk and milk products 2010-03-26 2010-06-01
GB/T 4789.19-
Microbiological examination of food hygiene Examination of
egg and egg products 2003-08-11 2004-01-01
GB/T 4789.20-
Microbiological examination of food hygiene Examination of
aquatic product foods 2003-08-11 2004-01-01
GB/T 4789.21-
Microbiological examination of food hygiene Examination of
frozen drinks and cold drinks 2003-08-11 2004-01-01
GB/T 4789.22-
Microbiological examination of food hygiene Examination of
flavourings 2003-08-11 2004-01-01
GB/T 4789.23-
Microbiological examination of food hygiene Examination of
cold dish and bean products 2003-08-11 2004-01-01
GB/T 4789.24-
Microbiological examination of food hygiene Examination of
candy, cake and preserved fruits 2003-08-11 2004-01-01
GB/T 4789.25-
Microbiological examination of food hygiene Examination of
wines 2003-08-11 2004-01-01
GB/T 4789.26-
Microbiological examination of food hygiene Examination of
commercial sterilization of canned food 2003-08-11 2004-01-01
GB/T 4789.27-
Microbiological examination of food hygiene. Examination of
residue of antibiotics in fresh milk 2008-11-21 2009-03-01
GB/T 4789.28-
Microbiological examination of food hygiene Staining
methods, culture mediums and reagents 2003-08-11 2004-01-01
GB/T 4789.29-
Microbiological examination of food hygiene Examination of
pseudomonas cocovenenans subsp. farinofermentans 2003-08-11 2004-01-01
GB 4789.30-2010 National food safety standard Food microbiological examination. Listeria monocytogenes 2010-03-26 2010-06-01
GB/T 4789.31-
Microbiological examination of food hygiene Examination of
salmonellae, shigellae, and diarrhoea causative Escherichia
coli by means of the diagnostic typing phage set for
enterobacteriaceae
GB/T 4789.32-
Microbiological examination for food hygiene rapid detection
of coliform bacteria 2002-06-13 2002-10-01
GB/T 4789.33-
Microbiological examination of food hygiene Examination of
cereal, fruit and vegetable 2003-08-11 2004-01-01
GB 4789.34-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Bifidobacterium 2012-05-17 2012-07-17
GB 4789.35-2010 National food safety standard Food microbiological examination. Lactic acid bacteria 2010-03-26 2010-06-01
GB/T 4789.36-
Microbiological examination of food hygiene. Examination of
Escherichia coli O157. H7/NM 2008-05-16 2008-11-01
GB/T 4789.37-
Microbiological examination of food hygiene. Examination of
Staphylococcus aureus 2008-11-21 2009-03-01
GB 4789.38-2012 National Food Safety Standard Microbiological Examination of ...