GB 4789.1-2010 English PDF

GB 4789.1-2010: National food safety standard -- Food microbiological examination: General guidelines
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GB 4789.1-2010
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard
Food Microbiological Examination.
General Guidelines
ISSUED ON. MARCH 26, 2010
IMPLEMENTED ON. JUNE, 1, 2010
Issued by. Ministry of Health of the People’s Republic of China
GB
Tips - GB 4789 Series (Not part of this Standard)
Standard ID Standard Name Issued Date
Enforced
Date New Version (Click to check)?
GB 4789.1-2010 National food safety standard Food microbiological examination. General guidelines 2010-03-26 2010-06-01
GB 4789.2-2010 National food safety standard Food microbiological examination. Aerobic plate count 2010-03-26 2010-06-01
GB 4789.3-2010 National food safety standard Food microbiological examination. Enumeration of coliforms 2010-03-26 2010-06-01
GB 4789.4-2010 National food safety standard Food microbiological examination. Salmonella 2010-03-26 2010-06-01
GB 4789.5-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Shigella 2012-05-17 2012-07-17
GB/T 4789.6-
Microbiological examination of food hygiene Examination of
diarrheogenic Escherichia coli 2003-08-11 2004-01-01
GB/T 4789.7-
Microbiological examination of food hygiene. Examination of
Vibrio parahaemolyticus 2008-05-16 2008-11-01
GB/T 4789.8-
Microbiological examination of food hygiene. Examination of
Yersinia enterocolitica 2008-11-21 2009-03-01
GB/T 4789.9-
Microbiological examination of food hygiene. Examination of
Campylobacter jejuni 2008-11-21 2009-03-01
GB 4789.10-2010 National food safety standard Food microbiological examination. Staphylococcus aureus 2008-11-21 2009-03-01
GB/T 4789.11-
Microbiological examination of food hygiene Examination of
streptococcus hemolyticus 2003-08-11 2004-01-01
GB/T 4789.12-
Microbiological examination of food hygiene Examination of
Clostridium botulinum and botulinus toxin 2003-08-11 2004-01-01
GB 4789.13-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Clostridium Perfringens 2012-05-17 2012-07-17
GB/T 4789.14-
Microbiological examination of food hygiene Examination of
Bacillus cereus 2003-08-11 2004-01-01
GB 4789.15-2010 National food safety standard Food microbiological examination. Enumeration of moulds and yeasts 2010-03-26 2010-06-01
GB/T 4789.16-
Microbiological examination of food hygiene Identification of
common mycotoxin producing fungi 2003-08-11 2004-01-01
GB/T 4789.17-
Microbiological examination of food hygiene Examination of
meat and meat products 2003-08-11 2004-01-01
GB 4789.18-2010 National food safety standard Food microbiological examination. Milk and milk products 2010-03-26 2010-06-01
GB/T 4789.19-
Microbiological examination of food hygiene Examination of
egg and egg products 2003-08-11 2004-01-01
GB/T 4789.20-
Microbiological examination of food hygiene Examination of
aquatic product foods 2003-08-11 2004-01-01
GB/T 4789.21-
Microbiological examination of food hygiene Examination of
frozen drinks and cold drinks 2003-08-11 2004-01-01
GB/T 4789.22-
Microbiological examination of food hygiene Examination of
flavourings 2003-08-11 2004-01-01
GB/T 4789.23-
Microbiological examination of food hygiene Examination of
cold dish and bean products 2003-08-11 2004-01-01
GB/T 4789.24-
Microbiological examination of food hygiene Examination of
candy, cake and preserved fruits 2003-08-11 2004-01-01
GB/T 4789.25-
Microbiological examination of food hygiene Examination of
wines 2003-08-11 2004-01-01
GB/T 4789.26-
Microbiological examination of food hygiene Examination of
commercial sterilization of canned food 2003-08-11 2004-01-01
GB/T 4789.27-
Microbiological examination of food hygiene. Examination of
residue of antibiotics in fresh milk 2008-11-21 2009-03-01
GB/T 4789.28-
Microbiological examination of food hygiene Staining
methods, culture mediums and reagents 2003-08-11 2004-01-01
GB/T 4789.29-
Microbiological examination of food hygiene Examination of
pseudomonas cocovenenans subsp. farinofermentans 2003-08-11 2004-01-01
GB 4789.30-2010 National food safety standard Food microbiological examination. Listeria monocytogenes 2010-03-26 2010-06-01
GB/T 4789.31-
Microbiological examination of food hygiene Examination of
salmonellae, shigellae, and diarrhoea causative Escherichia
coli by means of the diagnostic typing phage set for
enterobacteriaceae
GB/T 4789.32-
Microbiological examination for food hygiene rapid detection
of coliform bacteria 2002-06-13 2002-10-01
GB/T 4789.33-
Microbiological examination of food hygiene Examination of
cereal, fruit and vegetable 2003-08-11 2004-01-01
GB 4789.34-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Bifidobacterium 2012-05-17 2012-07-17
GB 4789.35-2010 National food safety standard Food microbiological examination. Lactic acid bacteria 2010-03-26 2010-06-01
GB/T 4789.36-
Microbiological examination of food hygiene. Examination of
Escherichia coli O157. H7/NM 2008-05-16 2008-11-01
GB/T 4789.37-
Microbiological examination of food hygiene. Examination of
Staphylococcus aureus 2008-11-21 2009-03-01
GB 4789.38-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Enumeration of Escherichia Coli 2012-05-17 2012-07-17
GB/T 4789.39-
Microbiological examination of food hygiene. Enumeration of
faecal coliforms 2008-11-21 2009-03-01
GB 4789.40-2010 National food safety standard Food microbiological examination. Enterobacter sakazakii 2010-03-26 2010-06-01
Table of Contents
Foreword ...7
1 Scope ...8
2 Normative references ...8
3 General laboratory requirements ...8
4 Sample collection ...10
5 Sample examination ...13
6 Biosafety and quality control...14
7 Recording and reporting ...14
8 Disposal of samples after examination ...14
Foreword
This Standard replaces GB/T 4789.1-2008 Microbiological Examination of Food
Hygiene – General Guidelines.
The main changes in this Standard over GB/T 4789.1-2008 are as follows.
－ Modify the standard name of both English and Chinese;
－ Modify the selection of examination methods.
The historical editions replaced by this Standard are as follows.
－ GB 4789.1-1984, GB 4789.1-1994, GB 4789.1-2003, and GB 4789.1-2008.
National food safety standard Food microbiological
examination. General guidelines
1 Scope
This Standard specifies the general principles and requirements for food microbiological
examination.
This Standard is applicable to the food microbiological examination.
2 Normative references
Documents referenced in this Standard are indispensable for application of this Standard.
For dated references, only the dated edition is applicable to this Standard. For undated
references, the latest edition (including all amendments) is applicable to this Standard.
3 General laboratory requirements
3.1 Environment
3.1.1 The laboratory environment shall not affect the accuracy of examination results.
3.1.2 The operation zone and the office zone of the laboratory shall be obviously
separated from each other.
3.1.3 The operation zone and general layout of the laboratory shall meet the requirements
for examination, and the laboratory layout shall adopt one-way work process to avoid
cross contamination.
3.1.4 Ambient temperature, humidity, luminance, noise, and cleanliness etc. of the
laboratory shall meet working requirements.
3.1.5 General sample examination shall be performed in clean zone (including super-clean
bench or clean laboratory), clean zone shall have visible markings.
3.1.6 Separation and identification of pathogenic microbes shall be performed in
laboratories of Biosafety level 2 (BSL-2).
3.2 Personnel
3.2.1 Examination personnel shall have related education or professional microbial
training experience, with appropriate qualifications, and can understand and
correctly implement the examination.
3.4.4 The items and materials used repeatedly (e.g. culture dish, suction tube, sucker, test
tube and inoculating loop, etc.) can be replaced with disposable items applicable to
the microbiological examination.
3.4.5 The storage environment for the examination appliances shall be kept dry and clean.
The sterilized and unsterilized appliances shall be stored separately and clearly
labeled.
3.4.6 The sterilization/disinfection temperature and duration shall be recorded for sterilized
examination appliances.
3.5 Culture medium and reagents
3.5.1 Culture medium
The preparation and quality control of culture medium shall follow the provisions of GB/T
4789.28.
3.5.2 Reagents
The quality and preparation of examination reagents shall be applicable to relevant
examination...
GB 4789.1-2010
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard
Food Microbiological Examination.
General Guidelines
ISSUED ON. MARCH 26, 2010
IMPLEMENTED ON. JUNE, 1, 2010
Issued by. Ministry of Health of the People’s Republic of China
GB
Tips - GB 4789 Series (Not part of this Standard)
Standard ID Standard Name Issued Date
Enforced
Date New Version (Click to check)?
GB 4789.1-2010 National food safety standard Food microbiological examination. General guidelines 2010-03-26 2010-06-01
GB 4789.2-2010 National food safety standard Food microbiological examination. Aerobic plate count 2010-03-26 2010-06-01
GB 4789.3-2010 National food safety standard Food microbiological examination. Enumeration of coliforms 2010-03-26 2010-06-01
GB 4789.4-2010 National food safety standard Food microbiological examination. Salmonella 2010-03-26 2010-06-01
GB 4789.5-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Shigella 2012-05-17 2012-07-17
GB/T 4789.6-
Microbiological examination of food hygiene Examination of
diarrheogenic Escherichia coli 2003-08-11 2004-01-01
GB/T 4789.7-
Microbiological examination of food hygiene. Examination of
Vibrio parahaemolyticus 2008-05-16 2008-11-01
GB/T 4789.8-
Microbiological examination of food hygiene. Examination of
Yersinia enterocolitica 2008-11-21 2009-03-01
GB/T 4789.9-
Microbiological examination of food hygiene. Examination of
Campylobacter jejuni 2008-11-21 2009-03-01
GB 4789.10-2010 National food safety standard Food microbiological examination. Staphylococcus aureus 2008-11-21 2009-03-01
GB/T 4789.11-
Microbiological examination of food hygiene Examination of
streptococcus hemolyticus 2003-08-11 2004-01-01
GB/T 4789.12-
Microbiological examination of food hygiene Examination of
Clostridium botulinum and botulinus toxin 2003-08-11 2004-01-01
GB 4789.13-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Clostridium Perfringens 2012-05-17 2012-07-17
GB/T 4789.14-
Microbiological examination of food hygiene Examination of
Bacillus cereus 2003-08-11 2004-01-01
GB 4789.15-2010 National food safety standard Food microbiological examination. Enumeration of moulds and yeasts 2010-03-26 2010-06-01
GB/T 4789.16-
Microbiological examination of food hygiene Identification of
common mycotoxin producing fungi 2003-08-11 2004-01-01
GB/T 4789.17-
Microbiological examination of food hygiene Examination of
meat and meat products 2003-08-11 2004-01-01
GB 4789.18-2010 National food safety standard Food microbiological examination. Milk and milk products 2010-03-26 2010-06-01
GB/T 4789.19-
Microbiological examination of food hygiene Examination of
egg and egg products 2003-08-11 2004-01-01
GB/T 4789.20-
Microbiological examination of food hygiene Examination of
aquatic product foods 2003-08-11 2004-01-01
GB/T 4789.21-
Microbiological examination of food hygiene Examination of
frozen drinks and cold drinks 2003-08-11 2004-01-01
GB/T 4789.22-
Microbiological examination of food hygiene Examination of
flavourings 2003-08-11 2004-01-01
GB/T 4789.23-
Microbiological examination of food hygiene Examination of
cold dish and bean products 2003-08-11 2004-01-01
GB/T 4789.24-
Microbiological examination of food hygiene Examination of
candy, cake and preserved fruits 2003-08-11 2004-01-01
GB/T 4789.25-
Microbiological examination of food hygiene Examination of
wines 2003-08-11 2004-01-01
GB/T 4789.26-
Microbiological examination of food hygiene Examination of
commercial sterilization of canned food 2003-08-11 2004-01-01
GB/T 4789.27-
Microbiological examination of food hygiene. Examination of
residue of antibiotics in fresh milk 2008-11-21 2009-03-01
GB/T 4789.28-
Microbiological examination of food hygiene Staining
methods, culture mediums and reagents 2003-08-11 2004-01-01
GB/T 4789.29-
Microbiological examination of food hygiene Examination of
pseudomonas cocovenenans subsp. farinofermentans 2003-08-11 2004-01-01
GB 4789.30-2010 National food safety standard Food microbiological examination. Listeria monocytogenes 2010-03-26 2010-06-01
GB/T 4789.31-
Microbiological examination of food hygiene Examination of
salmonellae, shigellae, and diarrhoea causative Escherichia
coli by means of the diagnostic typing phage set for
enterobacteriaceae
GB/T 4789.32-
Microbiological examination for food hygiene rapid detection
of coliform bacteria 2002-06-13 2002-10-01
GB/T 4789.33-
Microbiological examination of food hygiene Examination of
cereal, fruit and vegetable 2003-08-11 2004-01-01
GB 4789.34-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Bifidobacterium 2012-05-17 2012-07-17
GB 4789.35-2010 National food safety standard Food microbiological examination. Lactic acid bacteria 2010-03-26 2010-06-01
GB/T 4789.36-
Microbiological examination of food hygiene. Examination of
Escherichia coli O157. H7/NM 2008-05-16 2008-11-01
GB/T 4789.37-
Microbiological examination of food hygiene. Examination of
Staphylococcus aureus...