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GB 1903.54-2021: National Food Safety Standard - Food Nutrient Fortifier - Choline Bitartrate
GB 1903.54-2021
(National Food Safety Standard Food Nutrition Enhancer Choline Bitartrate)
National Standards of People's Republic of China
National Food Safety Standard
Food nutrient fortifier choline bitartrate
Released on 2021-09-07
Implementation on 2022-03-07
National Health Commission of the People's Republic of China
Issued by the State Administration for Market Regulation
National Food Safety Standard
Food nutrient fortifier choline bitartrate
1 Scope
This standard applies to trimethylamine, tartaric acid, and ethylene oxide as the main raw materials, which are concentrated (crystallized), separated and dried after the reaction.
Food nutrient fortifier choline bitartrate.
2 Chemical name, structural formula, molecular formula and relative molecular mass
2.1 Chemical name
(2-Hydroxyethyl) trimethylamine-tartrate
2.2 Structural formula
2.3 Molecular formula
C9H19NO7
2.4 Relative molecular mass
253.25 (according to.2018 international relative atomic mass)
3 Technical requirements
3.1 Sensory requirements
Sensory requirements should meet the requirements of Table 1.
Appendix A
Testing method
A.1 General provisions
When the reagents and water used in this standard do not indicate other requirements, they all refer to analytical reagents and the tertiary water specified in GB/T 6682.In the test
The standard solutions used, standard solutions for impurity determination, preparations and products are in accordance with GB/T 601, GB/T 602,
Prepared according to GB/T 603.The solution used in the test refers to the aqueous solution when the solvent is not specified.
A.2 Identification test
A.2.1 Infrared spectrum test
The potassium bromide smear method is used to measure the infrared absorption spectrum in accordance with GB/T 6040.The measured infrared spectrum should be in accordance with the standard of choline bitartrate.
The product map (see Appendix B) is consistent.
A.2.2 Precipitation reaction
A.2.2.1 Reagents and materials
Iodine solution. Weigh 14g of iodine, dissolve it in 100mL of water containing 36g of potassium iodide, add 3 drops of hydrochloric acid, and dilute with water to 1000mL,
Mix well.
A.2.2.2 Analysis steps
Weigh 0.5g sample and dissolve it in 2mL iodine solution, which immediately produced a reddish-brown precipitate. Add 5mL40g/L sodium hydroxide solution
Later, the precipitate was dissolved and the solution became clear yellow. Heating the solution produced a light yellow precipitate.
A.2.3 Color reaction
A.2.3.1 Reagents and materials
A.2.3.1.1 Potassium ferrocyanide solution. Weigh 2g of potassium ferrocyanide [K4Fe(CN)6·3H2O] and dissolve it in 100mL of water. This melt
The liquid is ready for use now.
A.2.3.1.2 Cobalt chloride solution. Weigh 2g of cobalt chloride (CoCl2·2H2O), dissolve it with 1mL of hydrochloric acid, and dilute to 100mL with water.
A.2.3.2 Analysis steps
Measure 1mL of 10mg/L sample solution, add 2mL of potassium ferrocyanide solution, and then add 2mL of cobalt chloride solution, it will be fresh immediately.
green.
A.3 Determination of choline bitartrate content (calculated on a dry basis)
A.3.1 Method summary
The sample uses glacial acetic acid as solvent, crystal violet as indicator, titrated with perchloric acid standard solution, and calculates the content based on the volume consumed.
A.3.2 Reagents and materials
A.3.2.1 Glacial acetic acid.
A.3.2.2 Perchloric acid standard titration solution. c(HClO4)=0.1mol/L.
A.3.2.3 Crystal violet indicator solution. 10g/L.
A.3.3 Analysis steps
Weigh about 0.5g of the sample (accurate to 0.0001g), add 50mL of glacial acetic acid, heat the steam bath to completely dissolve, and cool. Add 2 drops
Crystal violet indicator solution, titrate with perchloric acid standard titration solution (operate in a fume hood) until the solution turns green, and record the perchloric acid standard consumed
The volume of the solution. Do a blank test at the same time.
A.3.4 Result calculation
The mass fraction w1 of choline bitartrate content (calculated on a dry basis) is calculated according to formula (A.1).
w1=
c×(V1-V2)×M
1000×m×(1-w)×
100% (A.1)
Where.
c --- The concentration of perchloric acid standard titration solution, in moles per liter (mol/L);
V1 --- The volume of the perchloric acid standard solution consumed by the titration of the sample solution, in milliliter (mL);
V2 --- The volume of the perchloric acid standard solution consumed by the titration of the blank solution, in milliliter (mL);
M ---The molar mass of choline bitartrate, in grams per mole (g/mol), [M(C9H19NO7)=253.25];
1000---volume conversion factor;
m --- the mass of the sample, in grams (g);
w --- The moisture content of the sample, in grams per hundred grams (g/100g).
The test results are based on the arithmetic mean of the parallel determination results. Absolute difference between two independent determination results obtained under repeatability conditions
The value is not more than 0.5%.
A.4 Determination of ignition residue
A.4.1 Reagents and materials
sulfuric acid.
A.4.2 Instruments and equipment
A.4.2.1 Balance. Sensitivity is 0.1mg.
A.4.2.2 Crucible.
A.4.2.3 High temperature furnace.
A.4.2.4 Dryer.
A.4.3 Analysis steps
Take about 2g of the sample (accurate to 0.0001g), put it into a crucible that has been ignited to a constant weight, and slowly ignite it on the electric furnace until it is completely carbonized.
But to room temperature. Add 0.5mL sulfuric acid to moisten, heat at low temperature until sulfuric acid vapor completely escapes. Then move into the high temperature furnace, at 800℃±25℃
Burn down to completely ash. Take out the crucible, move it to a desiccator, let it cool to room temperature, weigh it accurately, and then burn it in a high-temperature furnace until it has a constant weight. Constant weight
The difference between the two weighings should not exceed 0.5mg. The carbonization operation is carried out in a fume hood.
A.4.4 Result calculation
The mass fraction w2 of the burning residue is calculated according to formula (A.2).
w2=
m1-m0
m ×100%
(A.2)
Where.
m1---The total mass of crucible and residue, in grams (g);
m0---the mass of the crucible, in grams (g);
m ---The mass of the sample, in grams (g).
The test results are based on the arithmetic mean of the parallel determination results. Absolute difference between two independent determination results obtained under repeatability conditions
The value is not more than 0.02%.
A.5 Determination of 1,4-dioxane
Measured in accordance with the method specified in GB/T 26388-2011.Among them, 7.2.2 is changed to "weigh 5.00g±0.01g sample in the headspace bottle
Then add 10.00mL±0.01mL standard solution A, immediately cap and seal, sonicate for 15-20min and then stand for 2h for chromatography
Determination"; 8.1.1a) is changed to "Column temperature. 80℃ constant temperature. Detector temperature. 250℃".