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GB 1886.352-2021 English PDF (GB1886.352-2021)

GB 1886.352-2021 English PDF (GB1886.352-2021)

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GB 1886.352-2021: National Food Safety Standard - Food Additive - ??-cyclodextrin

This Standard applies to the food additive ??-cyclodextrin that is prepared by enzymatic hydrolysis and purification of starch.
GB 1886.352-2021
GB
NATIONAL STANDARD OF THE
PEOPLE REPUBLIC OF CHINA
National Food Safety Standard - Food Additive -
??-cyclodextrin
ISSUED ON: SEPTEMBER 07, 2021
IMPLEMENTED ON: MARCH 07, 2022
Issued by: National Health Commission of the People's Republic of
China;
State Administration for Market Regulation.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Molecular formula and relative molecular mass ... 4
3 Technical requirements ... 4
Appendix A Inspection method ... 6
National Food Safety Standard - Food Additive - ??-
cyclodextrin
1 Scope
This Standard applies to the food additive ??-cyclodextrin that is prepared by enzymatic hydrolysis and purification of starch.
2 Molecular formula and relative molecular mass
2.1 Molecular formula
(C6H10O5)7
2.2 Relative molecular mass
1134.987 (according to the international relative atomic mass in 2018)
3 Technical requirements
3.1 Sensory requirements
Sensory requirements shall meet the requirements of Table 1.
3.2 Physical and chemical indicators
Physical and chemical indicators shall meet the requirements of Table 2. Appendix A
Inspection method
A.1 General provisions
The reagents and water used in this Standard refer to analytical reagents and grade-3 water in accordance with GB/T 6682, when other requirements are not indicated. The standard solutions used in the test, the standard solutions for impurity determination, the preparations and products, unless otherwise specified, shall be prepared in accordance with GB/T 601, GB/T 602 and GB/T 603. The solution used in the test refers to an aqueous solution, when it is not specified which solvent is used to prepare it.
A.2 Identification test
A.2.1 Specific rotation test
Weigh 1.00 g of the sample and dissolve it in 100 mL of water; measure it according to the method that is specified in GB/T 613. The specific rotation shall be +160?? ~ +164??.
A.2.2 Chromatographic test
In the ??-cyclodextrin content determination test, the retention time of the main peak of the sample solution chromatogram shall be consistent with the retention time of the main peak of the standard solution chromatogram.
A.3 Determination of ??-cyclodextrin content (on a dry basis)
A.3.1 Reagents and materials
A.3.1.1 Methanol: chromatographically pure.
A.3.1.2 Water: Grade-1 water specified in GB/T 6682.
A.3.1.3 ??-cyclodextrin standard substance: purity ???99.5%.
A.3.2 Instruments and equipment
High performance liquid chromatograph: equipped with a refractive index detector.
A.3.3 Reference chromatographic conditions
A.3.3.1 Chromatographic column: 10 ??m porous silica gel chromatographic column (??4.6 mm ?? 25 cm) with octadecylsilane-bonded silica gel as the filler, or other equivalent chromatographic column.
A.3.3.2 Mobile phase: water: methanol = 85: 15.
A.3.3.3 Column temperature: 30 ??C.
A.3.3.4 Detector temperature: 35 ??C.
A.3.3.5 Flow rate: 1.5 mL/min.
A.3.3.6 Injection volume: 10 ??L.
A.3.4 Analysis steps
A.3.4.1 Preparation of standard solution
Weigh 0.05 g of ??-cyclodextrin standard substance, accurate to 0.000 1 g; place it in a 10 mL volumetric flask; use the mobile phase to dissolve it and dilute to the mark; mix well. Use a 0.45 ??m microporous membrane for filtration before chromatographic analysis.
A.3.4.2 Preparation of sample solution
Weigh 0.05 g of the sample, accurate to 0.000 1 g; place it in a 10 mL volumetric flask; use the mobile phase to dissolve it and dilute to the mark; mix well. Use a 0.45 ??m microporous membrane for filtration before chromatographic
analysis.
A.3.5 Determination
Under the reference chromatographic conditions of A.3.3, respectively
determine the standard solution and sample solution. The system suitability means that, for 5 repeated injections of the standard solution, the relative standard deviation of the response area obtained is less than 2.0%.
A.3.6 Result calculation
The mass fraction w1 of the ??-cyclodextrin content (on a dry basis) is calculated according to Formula (A.1).
Where:
Au ?€? the peak area value of ??-cyclodextrin in the sample solution;

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