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GB 1886.176-2016 English PDF (GB1886.176-2016)

GB 1886.176-2016 English PDF (GB1886.176-2016)

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GB 1886.176-2016: Food additive -- Lactulose liquid

This standard applies to food additive isomerized lactose liquids, which are prepared from lactose as raw material by isomerization, with or without alkali under heating conditions, OR the solid products prepared by drying afterwards. 2 Structural formula, molecular formula, relative molecular mass of the main components 2.1 Structural formula 2.2 Molecular formula C12H22O11 2.3 Relative molecular mass 342.30 (according to 2011 international relative atomic mass) 3 Technical requirements 3.1 Sensory requirements Sensory requirements shall comply with Table 1. GB 1886.176-2016 www.ChineseStandard.net ? Buy True-PDF ? Auto-delivery. Page 5 of 9 Table 1 -- Sensory requirements Item Requirements Inspection methods Solid Liquid Color White or nearly white Colorless to dark yellow Take appropriate amounts of solid and liquid specimen; place them in white porcelain plates and colorimetric tubes respectively; observe their color and state under natural lightState Crystalline powder Transparent liquid 3.2 Physical and chemical indicators The physical and chemical indicators shall comply with the provisions of Table 2. Table 2 -- Physical and chemical indicators Item Requirements Inspection methods Solid Liquid Isomerized lactose content, w/% ???? 97.0 50.0 A.3 in Appendix A Fructose content, w/% ???? - 1.0 A.3 in Appendix A Galactose content, w/% ???? - 11.0 A.3 in Appendix A Epilactose content, w/% ???? - 4.0 A.3 in Appendix A Lactose content, w/% ???? - 6.0 A.3 in Appendix A pH a 3 ~ 7 GB/T 9724 Ignition residue, w/% ???? 0.5 GB/T 9741 Lead (Pb)/(mg/kg) ???? 1.0 GB 5009.75 or GB 5009.12 Arsenic (As)/(mg/kg) ???? 1.0 GB 5009.76 a The solid specimen shall be prepared into a 100 g/L solution. 3.3 Microbial indicators Microbiological indicators shall comply with the provisions of Table 3. Table 3 -- Microbiological indicators Item Indicators Inspection methods Total number of colonies/(CFU/g) ???? 1000 GB 4789.2 Escherichia coli/(MPN/g) and lt; 3.0 GB 4789.38 Salmonella/25 g Shall not be detected GB 4789.4...
GB 1886.176-2016
Food additive--Lactulose liquid
National Standards of People's Republic of China
National food safety standards
Food additive isomerized lactose solution
Published on.2016-08-31
2017-01-01 implementation
People's Republic
National Health and Family Planning Commission released
Foreword
This standard replaces GB 8816-1988 "Food additive isomerized lactose solution". Compared with GB 8816-1988, the main changes of this standard are as follows. --- Revised the sensory requirements;
--- Revised the isomerized lactose content, fructose content, galactose content, lactose content, arsenic (As) index requirements; --- Removed the specific gravity, refractive index, and indicator requirements of coliforms; --- Increased the content of the lactose content, pH, burning residue, Escherichia coli index requirements and testing methods; --- Increased the identification test method;
--- Revised the test method for isomerized lactose content, fructose content, galactose content, lactose content; --- The reference standard for the partial test method is adjusted to the latest released version. National food safety standards
Food additive isomerized lactose solution
1 Scope
This standard is applicable to isomerized lactose solution of food additive prepared by using lactose as raw material and being heated by alkali or without alkali isomerization. Or a solid product made by drying.
2 The structural formula, molecular formula and relative molecular mass of the main component 2.1 Structural formula
2.2 Molecular formula
C12H22O11
2.3 Relative molecular mass
342.30 (according to.2011 international relative atomic mass)
3 Technical requirements
3.1 Sensory requirements
Sensory requirements should be in accordance with Table 1.
Table 1 Sensory requirements
project
Claim
Solid liquid
Testing method
Color white or near white colorless to dark yellow
State crystalline powder transparent liquid
Take appropriate amount of solid and liquid samples in a white porcelain plate and a colorimetric tube, Observe its color and state under natural light
3.2 Physical and chemical indicators
Physical and chemical indicators should meet the requirements of Table 2. Table 2 Physical and chemical indicators
project
index
Solid liquid
Testing method
Isomerized lactose content, w/% ??? 97.0 50.0 Appendix A, A.3
Fructose content, w/% ??? - 1.0 Appendix A, A.3
Galactose content, w/% ??? - 11.0 A.3 in Appendix A
Epitrile content, w/% ??? - 4.0 Appendix A, A.3
Lactose content, w/% ??? - 6.0 Appendix A, A.3
pHa 3~7 GB/T 9724
Residue on ignition, w/% ??? 0.5 GB/T 9741
Lead (Pb)/(mg/kg) ??? 1.0 GB 5009.75 or GB 5009.12
Arsenic (As)/(mg/kg) ??? 1.0 GB 5009.76
a Solid sample should be formulated as a 100g/L solution.
3.3 Microbial indicators
Microbiological indicators should meet the requirements of Table 3.
Table 3 Microbial indicators
Project indicator test method
Total number of colonies/(CFU/g) ??? 1000 GB 4789.2
Escherichia coli/(MPN/g) < 3.0 GB 4789.38
Salmonella/25g shall not be detected GB 4789.4
Appendix A
Testing method
A.1 General provisions
The reagents and water used in this standard refer to the analytical reagents and the tertiary water specified in GB/T 6682 when no other requirements are specified. In trial Standard solutions, standard solutions for determination of impurities, preparations and products shall be in accordance with GB/T 601, GB/T 602 and Prepared in accordance with the provisions of GB/T 603. The solution used in the test refers to an aqueous solution when it is not indicated which solvent is used. A.2 Identification test
In the isomerized lactose content determination test, the main peak retention time of the sample solution chromatogram should be compared with the standard solution chromatogram (see Figure B.1). The main peak retention time is consistent.
A.3 Determination of isomerized lactose, fructose, galactose, elipide, lactose A.3.1 Reagents and materials
A.3.1.1 Water. Level 1 water in accordance with GB/T 6682.
A.3.1.2 Acetonitrile. chromatographically pure.
A.3.1.3 Isomerized lactose standard, fructose standard, galactose standard, eptimose standard, lactose standard. purity ??? 99.0%. A.3.1.4 Phosphate buffer. 1.15 g of anhydrous sodium dihydrogen phosphate was weighed and dissolved in water to a volume of 1000 mL. A.3.2 Instruments and equipment
A.3.2.1 High performance liquid chromatograph equipped with a refractive index detector, constant column temperature and vacuum degassing system. A.3.2.2 Column. Amino column with full porous silica as a filler (??4.6mm??15cm, particle size 3??m) or other equivalent color Column.
A.3.3 Reference chromatographic conditions
A.3.3.1 Mobile phase. acetonitrile. phosphate buffer = 82.18.
A.3.3.2 Flow rate. 1.3 mL/min.
A.3.3.3 Column temperature. 40 ??C ?? 1 ??C.
A.3.3.4 Injection volume. 20 ??L.
A.3.4 Analysis steps
A.3.4.1 Preparation of mixed standard solution
Weigh the fructose standard.200mg (accurate to 0.0001g), place it in a 10mL volumetric flask, add water to dissolve, and dilute to 10mL. Go to the fructose standard solution. Weighing 400 mg of isomerized lactose standard, 64 mg of galactose standard, 32 mg of ipose lactose standard, milk Sugar standard 52mg (accurate to 0.0001g) and fructose standard solution.200??L co-dissolved in a 10mL volumetric flask, add 4mL of water, fully soluble After the solution, 5 mL of acetonitrile was added, and the solution was allowed to warm to room temperature and then made up to 10 mL with water. A.3.4.2 Preparation of sample solution
Weigh the sample 2g ~ 4g (according to the dry basis, accurate to 0.0001g), placed in a 50mL volumetric flask. Add 20mL of water to fully dissolve After that, 25 mL of acetonitrile was added, and the solution was allowed to warm to room temperature and then made up to 50 mL with water. A.3.4.3 Determination
Under the A.3.3 reference chromatographic conditions, the mixed standard solution was injected into the chromatograph for determination, and the relative retention times of the different components were obtained. (See Table A.1). The prepared sample solution is injected, and the chromatographic peak of each component to be tested is qualitatively determined according to the retention time of the standard. The peak area calculates the mass fraction of each component to be tested. The applicability of the system is repeated injection of the standard solution twice, and the relative error of the obtained response area Should be less than 2.0%.
Table A.1 Relative retention time reference values for different components in a mixed standard solution Relative retention time of the component to be tested
Fructose 0.30
Galactose 0.42
Epidomide 0.85
Isomerized lactose 1.0
Lactose 1.1
A.3.4.4 Calculation of results
The mass fraction w1 of the component to be tested (isomerized lactose, fructose, galactose, elipideol, lactose) in the sample is based on the dry basis, according to formula (A.1) Calculation.
W1=
Au??cs
As??cu??
100% (A.1)
In the formula.
Au--the peak area response of a component in the sample solution;
Cs --- the concentration of a component in the standard solution, in milligrams per milliliter (mg/mL); As --- peak area response of a component in the standard solution;
Cu --- The concentration of a component of the sample solution in milligrams per milliliter (mg/mL). The test results are based on the arithmetic mean of the parallel determination results. Absolute difference between two independent determinations obtained under repeatability conditions The value is not more than 0.5%.
Appendix B
Liquid chromatogram
A reference liquid chromatogram of the isomerized lactose standard is shown in Figure B.1. Description.
1---fructose;
2---galactose;
3---Epidomide;
4---isomerized lactose;
5---Lactose.
Figure B.1 Reference chromatogram of isomerized lactose standards

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